Dai Yong, Lv Tianyu, Wang Kang, Huang Yuanshuai, Li Deping, Liu Jianjun
The Second Clinical Medical College, Jinan University, Shenzhen, Guangdong, R.P. China.
Saudi J Kidney Dis Transpl. 2008 Nov;19(6):952-9.
At present, the diagnosis of renal allograft rejection requires a renal biopsy. Clinical management of renal transplant patients would be improved if rapid, noninvasive and reliable biomarkers of rejection were available. This study is designed to determine whether such protein biomarkers can be found in renal-graft tissue proteomic approach. Orthotopic kidney transplantations were performed using Fisher (F344) or Lewis rats as donors and Lewis rats as recipients. Hence, there were two groups of renal transplant models: one is allograft (from F344 to Lewis rats); another is syngrafts (from Lewis to Lewis rats) serving as control. Renal tissues were collected 3, 7 and 14 days after transplantation. As many as 18 samples were analyzed by 2-D Electrophoresis and mass spectrometry (MALDI-TOF-TOF-MS). Eleven differentially expressed proteins were identified between groups. In conclusion, proteomic technology can detect renal tissue proteins associated with acute renal allograft rejection. Identification of these proteins as diagnostic markers for rejection in patients' urine or sera may be useful and non-invasive, and these proteins might serve as novel therapeutic targets that also help to improve the understanding of mechanism of renal rejection.
目前，同种异体肾移植排斥反应的诊断需要进行肾活检。如果能有快速、无创且可靠的排斥反应生物标志物，肾移植患者的临床管理将会得到改善。本研究旨在确定能否通过肾移植组织蛋白质组学方法找到此类蛋白质生物标志物。采用Fisher（F344）大鼠或Lewis大鼠作为供体，Lewis大鼠作为受体进行原位肾移植。因此，有两组肾移植模型：一组是同种异体移植（从F344大鼠到Lewis大鼠）；另一组是同基因移植（从Lewis大鼠到Lewis大鼠）作为对照。在移植后3天、7天和14天收集肾组织。多达18个样本通过二维电泳和质谱（基质辅助激光解吸电离飞行时间串联质谱）进行分析。在两组之间鉴定出11种差异表达蛋白。总之，蛋白质组学技术能够检测出与急性同种异体肾移植排斥反应相关的肾组织蛋白。将这些蛋白鉴定为患者尿液或血清中排斥反应的诊断标志物可能是有用且无创的，并且这些蛋白可能成为新的治疗靶点，也有助于增进对肾排斥反应机制的理解。
