与囊性纤维化儿童铜绿假单胞菌肺部感染相关的谷胱甘肽S-转移酶：初步研究
Glutathione S-transferases related to P. aeruginosa lung infection in cystic fibrosis children: preliminary study.
作者信息
Feuillet-Fieux Marie-Noëlle, Nguyen-Khoa Thao, Loriot Marie-Anne, Kelly Mairead, de Villartay Philippe, Sermet Isabelle, Verrier Patricia, Bonnefont Jean-Paul, Beaune Philippe, Lenoir Gérard, Lacour Bernard
机构信息
Assistance Publique-Hôpitaux de Paris, Necker-Enfants Malades Hospital, Laboratory of Biochemistry A, 149 rue de Sèvres, 75015 Paris, France.
出版信息
Clin Biochem. 2009 Jan;42(1-2):57-63. doi: 10.1016/j.clinbiochem.2008.09.116. Epub 2008 Oct 17.
OBJECTIVES
In cystic fibrosis (CF) children, we investigated the predictive impact of glutathione S-transferases (GST) activity and genotypes P1, M1 and T1, and antioxidant levels on stage-severity of Pseudomonas aeruginosa lung infection.
METHODS
GST activity was determined in whole blood by spectrophotometry, and GST genotypes by multiplex PCR RFLP for 36 CF and 9 control children. Levels of glutathione in erythrocyte and vitamins A, E and C in plasma were measured by HPLC.
RESULTS
No difference in GST activity and no relationship between GST activity and antioxidant levels were observed in CF children as compared to controls. However, GST activity was lower in CF children with severe clinical status and infection, and the frequency of GSTP1 wild type genotype AA, prevalent in uninfected CF children (75%), decreased in infected ones (33%).
CONCLUSION
GST activity and genotype could play an important role in modulating P. aeruginosa lung infection in CF patients.
目的
在囊性纤维化（CF）患儿中，我们研究了谷胱甘肽S-转移酶（GST）活性、P1、M1和T1基因型以及抗氧化剂水平对铜绿假单胞菌肺部感染分期严重程度的预测影响。
方法
采用分光光度法测定36例CF患儿和9例对照儿童全血中的GST活性，采用多重PCR-RFLP法测定GST基因型。通过高效液相色谱法测定红细胞中的谷胱甘肽水平以及血浆中维生素A、E和C的水平。
结果
与对照组相比，CF患儿的GST活性无差异，且GST活性与抗氧化剂水平之间无相关性。然而，临床状态严重且感染的CF患儿的GST活性较低，未感染CF患儿中普遍存在的GSTP1野生型基因型AA的频率（75%）在感染患儿中降低（33%）。
结论
GST活性和基因型可能在调节CF患者铜绿假单胞菌肺部感染中起重要作用。
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