Shiea Jentaie, Cho Yi-Tzu, Lin Yen-Hsiu, Chang Chin-Wen, Lo Li-Hua, Lee Yi-Chern, Ke Hung-Lung, Wu Wen-Jeng, Wu Deng-Chyang
Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, Taiwan.
Rapid Commun Mass Spectrom. 2008 Dec;22(23):3754-60. doi: 10.1002/rcm.3792.
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is used as an alternative method for the rapid diagnosis of albuminuria. This technique requires no further sample pretreatment than simply mixing the urine sample with a MALDI matrix and drying under ambient conditions. The resulting MALDI mass spectra reveal albumin ions having charges ranging from +1 to +5. The detection of albumin is possible using any of the three most common MALDI matrices - sinapinic acid (SA), 2,5-dihydroxybenzoic acid (2,5-DHB), or 4-hydroxy-alpha-cyanocinnamic acid (alpha-CHC). Using this analytical approach, the limit of detection for albumin in urine is 10(-6) M, approximately 5 to 10 times lower than that detectable through conventional chemical testing.
基质辅助激光解吸/电离飞行时间质谱（MALDI-TOF MS）被用作快速诊断蛋白尿的替代方法。该技术除了将尿液样本与MALDI基质简单混合并在环境条件下干燥外，无需进一步的样本预处理。所得的MALDI质谱显示出电荷范围从+1到+5的白蛋白离子。使用三种最常见的MALDI基质中的任何一种——芥子酸（SA）、2,5-二羟基苯甲酸（2,5-DHB）或4-羟基-α-氰基肉桂酸（α-CHC）——都可以检测到白蛋白。采用这种分析方法，尿液中白蛋白的检测限为10^(-6) M，比通过传统化学检测可检测到的下限低约5至10倍。
