Gijzen M, Lewinsohn E, Croteau R
Institute of Biological Chemistry, Washington State University, Pullman 99164-6340.
Arch Biochem Biophys. 1991 Sep;289(2):267-73. doi: 10.1016/0003-9861(91)90471-t.
Monoterpene cyclase activity is greatly increased in grand fir (Abies grandis) sapling stems in response to wounding and the composition of the cyclic olefin mixture generated differs from that produced constitutively as determined by radio gas-liquid chromatography. Cell-free extracts from wounded stems and from non-wounded controls were systematically compared for monoterpene cyclase activities following partial purification and separation of these enzymes by anion-exchange chromatography (Mono Q FPLC) and native PAGE. The increase in monoterpene cyclase activity following wounding represents both the apparent enhancement of constitutive cyclase activities and the appearance of novel cyclization enzymes that are absent in nonwounded controls. A pinene cyclase was shown to be the major wound-inducible enzyme directly responsible for oleoresin monoterpene formation and was tentatively identified as a 62-kDa protein by SDS-PAGE.
在大冷杉（Abies grandis）幼树茎中，单萜环化酶活性在受伤后显著增加，通过放射性气液色谱法测定，受伤后产生的环状烯烃混合物的组成与组成型产生的不同。在通过阴离子交换色谱法（Mono Q FPLC）和天然聚丙烯酰胺凝胶电泳对这些酶进行部分纯化和分离后，系统地比较了受伤茎和未受伤对照的无细胞提取物的单萜环化酶活性。受伤后单萜环化酶活性的增加既代表了组成型环化酶活性的明显增强，也代表了未受伤对照中不存在的新型环化酶的出现。已证明蒎烯环化酶是直接负责油树脂单萜形成的主要伤口诱导酶，并通过SDS-聚丙烯酰胺凝胶电泳初步鉴定为一种62 kDa的蛋白质。
