Mocanu Daniel, Kolesnychenko Aleksey, Aarts Sonja, Troost-Dejong Amanda, Pierik Anke, Vossenaar Erik, Stapert Henk
Philips Research, High Tech Campus, 5656 AE Eindhoven, The Netherlands.
J Biotechnol. 2009 Jan 15;139(2):179-85. doi: 10.1016/j.jbiotec.2008.10.001. Epub 2008 Oct 15.
The goal of this study is to assess the influence of mass transfer phenomena on DNA hybridization kinetics in a flow-through, porous microarray for fast molecular testing. We present a scaled mathematical model of coupled convection, diffusion and reaction in porous media, which was used to simulate hybridization kinetics and to analyze the influence of convective transport on the reaction rate. In addition to computer simulations, we also present experimental data of hybridization collected on our microarray system for different flow rates. The results reported in this paper provide for a better understanding of the interaction between reaction and mass transfer processes during flow-through hybridization and suggest criteria for system design and optimization.
本研究的目的是评估传质现象对用于快速分子检测的流通式多孔微阵列中DNA杂交动力学的影响。我们提出了一个多孔介质中对流、扩散和反应耦合的尺度数学模型，该模型用于模拟杂交动力学并分析对流传输对反应速率的影响。除了计算机模拟，我们还展示了在我们的微阵列系统上针对不同流速收集的杂交实验数据。本文报道的结果有助于更好地理解流通式杂交过程中反应与传质过程之间的相互作用，并为系统设计和优化提供标准。
