子宫内膜癌中胸苷磷酸化酶的胞浆活性

The cytosol activity of thymidine phosphorylase in endometrial cancer.

作者信息

Miszczak-Zaborska Elzbieta, Kubiak Robert, Bieńkiewicz Andrzej, Bartkowiak Jacek

机构信息

Department of Medical Biochemistry, Medical University of Lodz, 6/8 Mazowiecka Street, 92-215 Lodz, Poland.

出版信息

J Exp Clin Cancer Res. 2008 Nov 5;27(1):64. doi: 10.1186/1756-9966-27-64.

DOI:10.1186/1756-9966-27-64

PMID:18986516

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2588563/

Abstract

BACKGROUND

Thymidine phosphorylase (TP) is identical with platelet-derived endothelial cell growth factor (PD-ECGF) which promotes angiogenesis. The aim of this study was to evaluate the cytosol activity of TP in tumor samples from patients with endometrial cancer.

METHODS

The activity of TP was measured by the spectrophotometric method in the cytosol of endometrial tumor samples from 43 patients. Moreover, the expression of platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) protein and microvessel density (MD) were examined in the same endometrial tumor samples by immunohistochemical staining. Normal endometrium from 16 women, treated surgically due to nononcological reasons served as a control.A relationship between the cytosol TP activity, PD-ECGF/TP protein expression, MD and clinicopathologic features was investigated.

RESULTS

A significantly higher the cytosol TP activity, PD-ECGF/TP protein expression and MD was stated in malignant tumor samples when compared to the control (samples of normal endometrium). A positive statistically significant correlation between the cytosol enzyme activity and PD-ECGF/TP protein expression and MD was found, but weaker from the remaining ones between PD-ECGF/TP protein expression and MD was observed.Besides no correlation between the cytosol TP activity, PD-ECGF/TP protein expression as well as MD and grading or histopatological type of endometrial cancer was stated.

CONCLUSION

The cytosol TP activity in endometrial cancer is significantly higher than in normal endometrium, with no relation as to the stage and grade of tumors, but correlates with the PD-ECGF/TP protein expression and MD may therefore be associated with favorable prognosis in patients treated with chemo- or radiotherapy after surgery.

摘要

背景

胸苷磷酸化酶（TP）与促进血管生成的血小板衍生内皮细胞生长因子（PD - ECGF）相同。本研究的目的是评估子宫内膜癌患者肿瘤样本中TP的胞浆活性。

方法

采用分光光度法测定43例子宫内膜肿瘤样本胞浆中TP的活性。此外，通过免疫组织化学染色检测相同子宫内膜肿瘤样本中血小板衍生内皮细胞生长因子/胸苷磷酸化酶（PD - ECGF/TP）蛋白的表达和微血管密度（MD）。16名因非肿瘤原因接受手术治疗的女性的正常子宫内膜作为对照。研究了胞浆TP活性、PD - ECGF/TP蛋白表达、MD与临床病理特征之间的关系。

结果

与对照组（正常子宫内膜样本）相比，恶性肿瘤样本中的胞浆TP活性、PD - ECGF/TP蛋白表达和MD显著更高。发现胞浆酶活性与PD - ECGF/TP蛋白表达和MD之间存在统计学上的正相关，但PD - ECGF/TP蛋白表达与MD之间的相关性比其他两者之间的相关性弱。此外，未发现胞浆TP活性、PD - ECGF/TP蛋白表达以及MD与子宫内膜癌的分级或组织病理学类型之间存在相关性。