Suppr超能文献

大鼠器官发生过程中E-钙黏蛋白和N-钙黏蛋白共表达的免疫组织化学观察

Immunohistochemical observation of co-expression of E- and N-cadherins in rat organogenesis.

作者信息

Sakamoto Atsushi, Murata Kazumoto, Suzuki Hideto, Yatabe Megumi, Kikuchi Motoshi

机构信息

Division of Forensic Medicine, Center for Community Medicine, Jichi Medical University School of Medicine, Tochigi, Japan.

出版信息

Acta Histochem Cytochem. 2008 Oct 29;41(5):143-7. doi: 10.1267/ahc.08026. Epub 2008 Oct 10.

Abstract

Cadherins are a family of transmembrane glycoproteins that mediate cell-to-cell adhesion. Isoforms, including E- and N-cadherin, have been identified and shown to regulate morphogenesis through homophilic binding. In the ontogeny, the expressions of E- and N-cadherin change spatiotemporally, and the changes in cadherin isoforms, called cadherin switching, impact the mechanical adhesion of cells. Furthermore, cadherin functions as a receptor that transfers information from outside to inside cells, and in terms of switching, it affects cell phenotypes. To observe the expression patterns of E- and N-cadherins during embryogenesis and to identify cells that transiently coexpress both cadherins, we employed a recently developed immunohistochemical double staining technique in rat fetuses. At embryonic day 9, embryonic ectodermal cells more dominantly expressed E-cadherin, while mesodermal cells more dominantly expressed N-cadherin. At embryonic day 10, the expression pattern of E-cadherin in the surface ectoderm and endoderm and that of N-cadherin in the neuroectoderm were established. After embryonic day 10, unique co-expression of E- and N-cadherin was observed in primordia, such as the bulbus cordis, otic pit, notochord, and Rathke's pouch. In the present study, it was possible to visualize the expression patterns of E- and N-cadherin during early fetal development, which enabled us to morphologically clarify cadherin switching.

摘要

钙黏蛋白是一类介导细胞间黏附的跨膜糖蛋白。已鉴定出包括E-钙黏蛋白和N-钙黏蛋白在内的多种异构体,并表明它们通过同源性结合来调节形态发生。在个体发育过程中,E-钙黏蛋白和N-钙黏蛋白的表达随时间和空间变化,而钙黏蛋白异构体的这种变化,即所谓的钙黏蛋白转换,会影响细胞的机械黏附。此外,钙黏蛋白作为一种受体,可将信息从细胞外传递到细胞内,就转换而言,它会影响细胞表型。为了观察胚胎发育过程中E-钙黏蛋白和N-钙黏蛋白的表达模式,并识别同时短暂共表达这两种钙黏蛋白的细胞,我们在大鼠胎儿中采用了一种最近开发的免疫组织化学双重染色技术。在胚胎第9天,胚胎外胚层细胞更主要地表达E-钙黏蛋白,而中胚层细胞更主要地表达N-钙黏蛋白。在胚胎第10天,表面外胚层和内胚层中E-钙黏蛋白的表达模式以及神经外胚层中N-钙黏蛋白的表达模式得以确立。胚胎第10天后,在原基中观察到E-钙黏蛋白和N-钙黏蛋白独特的共表达,如心球、耳窝、脊索和拉特克囊。在本研究中,能够可视化早期胎儿发育过程中E-钙黏蛋白和N-钙黏蛋白的表达模式,并使我们能够从形态学上阐明钙黏蛋白转换。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/142a/2576505/321fdba891e8/AHC08026f01.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验