Tsuzuki Saki, Wada Akira, Ito Yoshihiro
Nano Medical Engineering Laboratory, RIKEN, 2-1 Hirosawa, Wako-shi, Saitama 351-0198, Japan.
Biotechnol Bioeng. 2009 Feb 15;102(3):700-7. doi: 10.1002/bit.22102.
The photo-immobilization technique is useful for immobilization of various biomolecules on assorted material surfaces, independent of the organic functional groups that may be present. Here, we report a convenient new photo-immobilization technique that was developed by combining a nonbiofouling polymer containing polyethylene glycol and a photoreactive crosslinker for surface plasmon resonance (SPR) and quartz crystal microbalance (QCM) measurements. By this method, nonspecific interactions were reduced and various types of molecules, bovine serum albumin, heparin, dsDNA, phosphatidylserine, Tobacco Mosaic Virus, and norfloxacine, were immobilized on an alkane thiol-modified gold surface by a single method. The interactions of photo-immobilized biomolecules and their corresponding antibodies were investigated by SPR and QCM. In addition, SPR imaging was possible using the present method.
光固定技术可用于将各种生物分子固定在各种材料表面，而与可能存在的有机官能团无关。在此，我们报道了一种便捷的新型光固定技术，该技术是通过将含聚乙二醇的抗生物污染聚合物与用于表面等离子体共振（SPR）和石英晶体微天平（QCM）测量的光反应性交联剂相结合而开发的。通过这种方法，减少了非特异性相互作用，并且通过单一方法将各种类型的分子，即牛血清白蛋白、肝素、双链DNA、磷脂酰丝氨酸、烟草花叶病毒和诺氟沙星固定在烷硫醇修饰的金表面上。通过SPR和QCM研究了光固定生物分子与其相应抗体之间的相互作用。此外，使用本方法还可以进行SPR成像。
