Division of Wood Chemistry and Pulp Technology, Department of Fibre and Polymer Technology, Royal Institute of Technology, KTH, 10044 Stockholm, Sweden.
Biomacromolecules. 2008 Dec;9(12):3378-82. doi: 10.1021/bm800704k.
Dehydrogenation polymers (DHPs or synthetic lignins) were synthesized from coniferyl alcohol by enzymatic oxidation in the presence of ascorbic acid to study the potential effects of an antioxidant upon their structure. Specific interunit substructures (beta-O-4', beta-beta', and beta-5') were quantified by 13C NMR, which showed how ascorbic acid altered their amounts compared with control syntheses without this antioxidant, especially by increasing the amount of beta-O-4' substructures. The effect of ascorbic acid increased with its concentration. Surprisingly, no influence on the sizes of the synthetic lignins, as determined by size exclusion chromatography, was observed. The chemistry of this antioxidant effect during dehydrogenative polymerization and the potential biological significance (cell wall lignification) of these observations are discussed.
脱氢聚合物（DHPs 或合成木质素）是由松柏醇在抗坏血酸存在下通过酶氧化合成的，旨在研究抗氧化剂对其结构的潜在影响。通过 13C NMR 定量分析了特定的单元间亚结构（β-O-4'、β-β'和β-5'），结果表明抗坏血酸如何与没有这种抗氧化剂的对照合成物相比改变了它们的含量，特别是通过增加β-O-4'亚结构的含量。抗氧化剂的作用随着其浓度的增加而增加。令人惊讶的是，通过尺寸排阻色谱法观察到，对合成木质素的大小没有影响。讨论了这种抗氧化剂在脱氢聚合过程中的化学作用以及这些观察结果的潜在生物学意义（细胞壁木质化）。
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