Kobayashi Satoshi, Ise Hirohiko, Takahashi Masafumi, Goto Mitsuaki, Akaike Toshihiro, Ikeda Uichi
Division of Cardiovascular Medicine, Department of Organ Regeneration, Shinshu University Graduate School of Medicine, 3-1-1 Asahi, Matsumoto, Nagano 390-8621, Japan.
Biomaterials. 2009 Feb;30(4):574-82. doi: 10.1016/j.biomaterials.2008.10.002. Epub 2008 Nov 6.
Bone marrow implantation (BMI) has been performed clinically for the treatment of ischemic cardiovascular diseases. To achieve BMI effectively, accumulation of many bone marrow cells (BMCs) in an infarcted area of the myocardium is important. Previously, we reported that cardiomyocytes show strong interaction with N-acetylglucosamine (GlcNAc) and they can take up GlcNAc-conjugated liposomes. Thus, we examined whether GlcNAc-coated BMCs exhibit strong interaction with cardiomyocytes. The cell surface of BMCs was coated with GlcNAc without causing cell injury by GlcNAc-lipophilic polymers. It was found that the GlcNAc-coated BMCs exhibited strong interaction with cardiomyocytes. At 7 days of coculturing the GlcNAc-coated BMCs with cardiomyocytes, BMC-derived cardiomyocytes were generated. The number of BMC-derived cardiomyocytes was higher following coculture with GlcNAc-coated BMCs than following coculture with uncoated and maltose (MA)-coated BMCs. In this study, we demonstrated that the surface coating of BMCs with GlcNAc can be performed easily by using GlcNAc-lipophilic polymers and that GlcNAc-coated BMCs exhibited strong interaction with cardiomyocytes. Therefore, we think that cell surface coating with GlcNAc would help promote accumulation of BMCs in the infarcted area of the myocardium and that this accumulation would be helpful in the treatment of ischemic cardiovascular diseases with BMI.
骨髓植入术（BMI）已在临床上用于治疗缺血性心血管疾病。为了有效地实现BMI，使许多骨髓细胞（BMC）在心肌梗死区域积聚很重要。此前，我们报道心肌细胞与N-乙酰葡糖胺（GlcNAc）表现出强烈相互作用，并且它们可以摄取GlcNAc偶联的脂质体。因此，我们研究了GlcNAc包被的BMC是否与心肌细胞表现出强烈相互作用。通过GlcNAc-亲脂性聚合物在不引起细胞损伤的情况下用GlcNAc包被BMC的细胞表面。发现GlcNAc包被的BMC与心肌细胞表现出强烈相互作用。在将GlcNAc包被的BMC与心肌细胞共培养7天时，产生了源自BMC的心肌细胞。与GlcNAc包被的BMC共培养后，源自BMC的心肌细胞数量高于与未包被和麦芽糖（MA）包被的BMC共培养后的数量。在本研究中，我们证明使用GlcNAc-亲脂性聚合物可以轻松地用GlcNAc对BMC进行表面包被，并且GlcNAc包被的BMC与心肌细胞表现出强烈相互作用。因此，我们认为用GlcNAc进行细胞表面包被将有助于促进BMC在心肌梗死区域的积聚，并且这种积聚将有助于用BMI治疗缺血性心血管疾病。
