来自球形链霉菌新生霉素生物合成簇的O-氨甲酰基转移酶NovN的结晶及初步X射线分析。
Crystallization and preliminary X-ray analysis of the O-carbamoyltransferase NovN from the novobiocin-biosynthetic cluster of Streptomyces spheroides.
作者信息
Gómez García Inmaculada, Freel Meyers Caren L, Walsh Christopher T, Lawson David M
机构信息
Department of Biological Chemistry, John Innes Centre, Norwich NR4 7UH, England.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1000-2. doi: 10.1107/S1744309108030145. Epub 2008 Oct 25.
Abstract
Crystals of recombinant NovN, an O-carbamoyltransferase from Streptomyces spheroides, were grown by vapour diffusion. The protein crystallized in two different crystal forms. Crystal form I belonged to space group C2 and native data were collected to 2.9 A resolution in-house. Crystal form II had I-centred orthorhombic symmetry and native data were recorded to a resolution of 2.3 A at a synchrotron. NovN catalyses the final step in the biosynthesis of the aminocoumarin antibiotic novobiocin that targets the essential bacterial enzyme DNA gyrase.
摘要
来自球形链霉菌的O-氨甲酰基转移酶重组NovN的晶体通过气相扩散法生长。该蛋白质以两种不同的晶体形式结晶。晶体形式I属于空间群C2，内部收集到分辨率为2.9 Å的天然数据。晶体形式II具有I心正交对称，在同步加速器上记录到分辨率为2.3 Å的天然数据。NovN催化氨基香豆素抗生素新生霉素生物合成的最后一步，新生霉素靶向必需的细菌酶DNA促旋酶。
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