Jain Ankur, Ziegler Jörg, Liscombe David K, Facchini Peter J, Tucker Paul A, Panjikar Santosh
EMBL Hamburg Outstation, c/o DESY, Notkestrasse 85, D-22603 Hamburg, Germany.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Nov 1;64(Pt 11):1066-9. doi: 10.1107/S1744309108033046. Epub 2008 Oct 31.
A cDNA from the plant Thalictrum flavum encoding pavine N-methyltransferase, an enzyme belonging to a novel class of S-adenosylmethionine-dependent N-methyltransferases specific for benzylisoquinoline alkaloids, has been heterologously expressed in Escherichia coli. The enzyme was purified using affinity and gel-filtration chromatography and was crystallized in space group P2(1). The structure was solved at 2.0 A resolution using a xenon derivative and the single isomorphous replacement with anomalous scattering method.
来自植物黄唐松草的一个编码罂粟碱N-甲基转移酶的cDNA已在大肠杆菌中进行了异源表达,该酶属于一类新型的对苄基异喹啉生物碱具有特异性的依赖S-腺苷甲硫氨酸的N-甲基转移酶。该酶通过亲和色谱和凝胶过滤色谱进行纯化,并在空间群P2(1)中结晶。使用氙衍生物并采用单对映体置换与反常散射法在2.0 Å分辨率下解析了其结构。