电通透人血小板中Ca2(+)驱动的[3H]花生四烯酸释放显示对MgATP2-有绝对需求。
Ca2(+)-driven [3H]arachidonate release in electropermeabilized human platelets shows an absolute requirement for MgATP2-.
作者信息
Patel S, Scrutton M C
机构信息
Division of Biomolecular Sciences, King's College, London, U.K.
出版信息
Biochem J. 1991 Feb 1;273 ( Pt 3)(Pt 3):561-4. doi: 10.1042/bj2730561.
Abstract
Addition of micromolar Ca2+ to electropermeabilized human platelets which had been pre-labelled with [3H]arachidonate causes release of 3H only when millimolar concentrations of a nucleoside triphosphate, e.g. ATP, are present in the incubation medium. Addition of millimolar Ca2+ in the absence of ATP, or preincubation with ATP before addition of micromolar Ca2+, fails to cause a significant increase in 3H release. Purine nucleotides are more effective than pyrimidine nucleotides in activating Ca2(+)-driven 3H release. This activation does not appear to involve phosphate transfer, since metabolically stable analogues of ATP, e.g. the beta gamma-imido analogue, are effective in promoting 3H release.
摘要
向预先用[3H]花生四烯酸标记的电通透人血小板中添加微摩尔浓度的Ca2+，只有当孵育介质中存在毫摩尔浓度的核苷三磷酸（如ATP）时，才会导致3H释放。在没有ATP的情况下添加毫摩尔浓度的Ca2+，或在添加微摩尔浓度的Ca2+之前用ATP预孵育，均不会导致3H释放显著增加。嘌呤核苷酸在激活Ca2+驱动的3H释放方面比嘧啶核苷酸更有效。这种激活似乎不涉及磷酸转移，因为ATP的代谢稳定类似物（如βγ-亚氨基类似物）在促进3H释放方面是有效的。
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Thrombin and C-kinase activators potentiate calcium-stimulated arachidonic acid release in human platelets.凝血酶和C激酶激活剂可增强钙离子刺激的人血小板花生四烯酸释放。
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