College of Life Science, Hebei University, Wusi East Road 180, Baoding, 071002, Hebei Province, China,
Cytotechnology. 2008 Feb;56(2):113-21. doi: 10.1007/s10616-008-9132-5. Epub 2008 Feb 13.
The transforming characteristics of the membrane complex in spermatogenesis of Fenneropenaeus chinensis have been studied by using transmission electron microscopy. Two types of membrane complex have been investigated based on their sources: one originating from nucleus and the other from cytoplasm. The first one, consisted of annular structures, monolayer membrane blebs, and double or multi-lamellar membrane vesicles, emerges in the primary spermatocyte, then diffuses with the nuclear membrane and finally enters the cytoplasm. This type of membrane complex seems to play an important role in the materials transfusion from nucleus to cytoplasm, and it mainly exists inside the primary spermatocyte with some inside the secondary spermatocyte. The latter, originated from cytoplasm, is formed during the anaphase of spermiogenesis. It also exists in mature sperm, locating at both sides of the nucleus under the acrosomal cap. This type of membrane complex mainly comprises rings of convoluted membrane pouches, together with mitochondria, annular lamina bodies, fragments of endoplasmic reticulum, nuclear membrane and some nuclear particles. It releases vesicles and particles into the acrosomal area during the formation of the perforatorium, suggesting a combined function of the endoplasmic reticulum, mitochondria and Golgi's mechanism.
采用透射电镜技术研究了中国对虾精子发生过程中膜复合体的转化特征。根据其来源，将两种类型的膜复合体分为核来源和细胞质来源：一种起源于核，另一种起源于细胞质。第一种由环形结构、单层膜泡和双或多层膜小泡组成，出现在初级精母细胞中，然后与核膜扩散，最终进入细胞质。这种膜复合体似乎在核质物质传递中起着重要作用，主要存在于初级精母细胞内，部分存在于次级精母细胞内。后者起源于细胞质，在精子形成的后期形成。它也存在于成熟精子中，位于顶体帽下核的两侧。这种膜复合体主要由卷曲膜囊环、线粒体、环层小体、内质网片段、核膜和一些核颗粒组成。在顶体形成过程中，它将小泡和颗粒释放到顶体区域，提示内质网、线粒体和高尔基体机制的联合功能。
