Choju Medical Institute, Fukushimura Hospital, Noyori-cho, Toyohashi, 441-8124, Japan,
Cytotechnology. 2008 Mar;56(3):209-17. doi: 10.1007/s10616-008-9150-3. Epub 2008 Jun 5.
From unfractionated embryonic mice liver cells, appreciable amount of spherical bodies containing nestin-positive cells were generated in the presence of neuronal growth factors. Following cultivation on poly-D: -lysine/laminin-coated slips, approximately 70% of the cells expressed neuronal markers, and 16% had long processes. Functional analysis of these long-process-bearing cells with the whole-cell patch clamp method showed an inward current in response to glutamate, GABA, and serotonin as the neuronal characteristics. Furthermore, regenerating liver in adult mice also contained nestin-positive cells to the same extent as fetal liver. Regenerating liver could have potential as a source of neural cells for autologous transplantation.
从未分馏的胚胎小鼠肝细胞中，在神经元生长因子的存在下，产生了含有巢蛋白阳性细胞的大量球体。在聚-D: -赖氨酸/层粘连蛋白包被的载玻片上培养后，约 70%的细胞表达神经元标志物，16%的细胞具有长突起。用全细胞膜片钳方法对这些具有长突起的细胞进行功能分析表明，它们具有对谷氨酸、GABA 和 5-羟色胺的内向电流反应，这是神经元的特征。此外，成年小鼠的再生肝中也含有与胎肝相同程度的巢蛋白阳性细胞。再生肝可能是自体移植神经细胞的潜在来源。
