Chemical & Biomolecular Engineering, Georgia Institute of Technology, 315 Ferst Drive, IBB Building, Room 1306, Atlanta, GA, 30332, USA.
Cytotechnology. 2006 Jul;51(3):159-70. doi: 10.1007/s10616-006-9025-4. Epub 2006 Nov 16.
This study investigates the effect of alginate/poly-L: -lysine/alginate (APA) encapsulation on the insulin secretion dynamics exhibited by an encapsulated cell system. Experiments were performed with the aid of a home-built perfusion apparatus providing a 1 min temporal resolution. Insulin profiles were measured from: (i) murine insulinoma betaTC3 cells encapsulated in calcium alginate/poly-L: -lysine/alginate (APA) beads generated with high guluronic (G) or high mannuoric (M) content alginate, and (ii) murine insulinoma betaTC-tet cells encapsulated in high M APA beads and propagated in the presence and absence of tetracycline. Results show that encapsulation in APA beads did not affect the insulin secretion profile shortly post-encapsulation. However, remodeling of the beads due to cell proliferation affected the insulin secretion profiles; and inhibiting remodeling by suppressing cell growth preserved the secretion profile. The implications of these findings regarding the in vivo function of encapsulated insulin secreting cells are discussed.
本研究考察了藻酸盐/聚-L：-赖氨酸/藻酸盐（APA）包封对包封细胞系统表现出的胰岛素分泌动力学的影响。实验借助于一个内部构建的灌注装置进行，该装置提供了 1 分钟的时间分辨率。从以下方面测量胰岛素图谱：（i）在具有高古洛糖醛酸（G）或高甘露糖醛酸（M）含量藻酸盐的钙藻酸盐/聚-L：-赖氨酸/藻酸盐（APA）珠中包封的鼠胰岛素瘤βTC3 细胞，和（ii）在存在和不存在四环素的情况下在高 M APA 珠中包封并增殖的鼠胰岛素瘤βTC-tet 细胞。结果表明，APA 珠中的包封在包封后不久不会影响胰岛素的分泌模式。然而，由于细胞增殖导致的珠的重塑会影响胰岛素的分泌模式；通过抑制细胞生长抑制重塑可以保留分泌模式。讨论了这些发现对体内包封的胰岛素分泌细胞功能的影响。
