Graduate School of Systems Life Sciences, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka, 812-8581, Japan.
Cytotechnology. 2007 Dec;55(2-3):61-70. doi: 10.1007/s10616-007-9073-4. Epub 2007 Jul 20.
Catalyser-21(TM) is a mineral water derived from natural leaf soil containing various organic and inorganic substances. Previous reports suggested a possibility that Catalyser-21(TM) has antioxidative potential and could inhibit angiogenesis and cancer cell invasiveness. Angiogenesis is a prerequisite for cancer cells to spread to surrounding tissues. Vascular endothelial growth factor (VEGF) is a major angiogenic factor in the formation of blood capillaries by cancer cells to supply nutrients and oxygen for their sustained growth. Matrix metalloproteinase-2 (MMP-2) is another key enzyme for cancer cell metastasis. To assess the anti-angiogenic activity of Catalyser-21(TM), we first examined cell viability using a human cervical cancer cell line, HeLa, and a fibrosarcoma cell line, HT1080. The results showed that Catalyser-21(TM) decreased the viability of both cell types in a dose-dependent manner. Flow cytometric analysis proved that Catalyser-21(TM) scavenges intracellular H(2)O(2) in both cell types. RT-PCR demonstrated that both VEGF and MMP-2 gene transcription was suppressed after Catalyser-21(TM) treatment. Both Matrigel and tubule formation experiments showed an effect of Catalyser-21(TM). These results suggest that Catalyser-21(TM) has potential as an anti-tumor agent.
Catalyser-21(TM) 是一种源自天然叶土的矿泉水，含有各种有机和无机物质。先前的报告表明，Catalyser-21(TM) 可能具有抗氧化潜力，可以抑制血管生成和癌细胞侵袭。血管生成是癌细胞扩散到周围组织的前提。血管内皮生长因子 (VEGF) 是癌细胞形成毛细血管的主要血管生成因子，为其持续生长提供营养和氧气。基质金属蛋白酶-2 (MMP-2) 是癌细胞转移的另一个关键酶。为了评估 Catalyser-21(TM) 的抗血管生成活性，我们首先使用人宫颈癌细胞系 HeLa 和纤维肉瘤细胞系 HT1080 检查了细胞活力。结果表明，Catalyser-21(TM) 以剂量依赖的方式降低了两种细胞类型的活力。流式细胞术分析证明 Catalyser-21(TM) 清除了两种细胞类型中的细胞内 H(2)O(2)。RT-PCR 表明 Catalyser-21(TM) 处理后 VEGF 和 MMP-2 基因转录均受到抑制。Matrigel 和小管形成实验均显示 Catalyser-21(TM) 的作用。这些结果表明 Catalyser-21(TM) 具有作为抗肿瘤剂的潜力。
