电穿孔：应用的武器库。
Electroporation: an arsenal of application.
机构信息
Department of Biological Science and Biotechnology, Life Science School, Sun Yat-Sen (ZhongShan) University, P.O. Box A075#, XinGangXi Road 135, HaiZhu District, 510275, Guangzhou, China,
出版信息
Cytotechnology. 2007 Jun;54(2):71-6. doi: 10.1007/s10616-007-9082-3. Epub 2007 Jun 16.
Abstract
Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed, whose most important point is that it adopts low energy and rectangular pulse that could obtain high transfection efficiency and low damage to the embryo. This paper reviewed on the pool of application: from lab works to human clinical treatments.
摘要
电穿孔是一种利用人工电场诱导纳米级细胞膜孔，将外源物质递送到细胞质中的方法。现在它被广泛用于分子转移，特别是在分子实验和遗传方面。近年来，胚胎的现代电穿孔技术得到了发展，其最重要的一点是采用低能量和矩形脉冲，从而可以获得高转染效率和低胚胎损伤。本文综述了其应用范围：从实验室工作到人类临床治疗。
相似文献
1
Electroporation: an arsenal of application.电穿孔：应用的武器库。
Cytotechnology. 2007 Jun;54(2):71-6. doi: 10.1007/s10616-007-9082-3. Epub 2007 Jun 16.
2
High efficiency, site-specific transfection of adherent cells with siRNA using microelectrode arrays (MEA).使用微电极阵列（MEA）对贴壁细胞进行高效、位点特异性的siRNA转染。
J Vis Exp. 2012 Sep 13(67):e4415. doi: 10.3791/4415.
3
Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA.基于电穿孔体积和质粒 DNA 电泳迁移率的基因电转效率的数值研究。
Biomed Eng Online. 2018 Jun 18;17(1):80. doi: 10.1186/s12938-018-0515-3.
4
Gain- and loss-of-function approaches in the chick embryo.鸡胚中的功能获得和功能丧失方法。
Methods Cell Biol. 2008;87:237-56. doi: 10.1016/S0091-679X(08)00212-4.
5
Response characteristics and optimization of electroporation: simulation based on finite element method.电穿孔的响应特性和优化：基于有限元法的模拟。
Electromagn Biol Med. 2021 Jul 3;40(3):321-337. doi: 10.1080/15368378.2021.1951484. Epub 2021 Jul 18.
6
Avoiding the side effects of electric current pulse application to electroporated cells in disposable small volume cuvettes assures good cell survival.避免在一次性小容量比色皿中对电穿孔细胞施加电流脉冲产生的副作用可确保细胞良好存活。
Cell Mol Biol Lett. 2017 Jan 13;22:1. doi: 10.1186/s11658-016-0030-0. eCollection 2017.
7
Gene transfer to skeletal muscle by site-specific delivery of electroporation and ultrasound.电穿孔和超声定点传递将基因转移到骨骼肌。
Biochem Biophys Res Commun. 2012 Jul 27;424(2):203-7. doi: 10.1016/j.bbrc.2012.06.090. Epub 2012 Jun 26.
8
Correlation between the loss of intracellular molecules and cell viability after cell electroporation.细胞电穿孔后细胞内分子的损失与细胞活力的相关性。
Bioelectrochemistry. 2020 Oct;135:107550. doi: 10.1016/j.bioelechem.2020.107550. Epub 2020 May 13.
9
Microtrap array on a chip for localized electroporation and electro-gene transfection.芯片上的微阱阵列用于局部电穿孔和电基因转染。
Bioelectrochemistry. 2022 Oct;147:108197. doi: 10.1016/j.bioelechem.2022.108197. Epub 2022 Jun 28.
10
Electroporation of subcutaneous mouse tumors by rectangular and trapezium high voltage pulses.通过矩形和梯形高压脉冲对小鼠皮下肿瘤进行电穿孔。
Bioelectrochemistry. 2004 Apr;62(1):83-93. doi: 10.1016/j.bioelechem.2003.11.001.
引用本文的文献
1
In vitro and in vivo gene transfer in the cloudy catshark Scyliorhinus torazame.在虎斑乌鲨 Scyliorhinus torazame 中进行的体外和体内基因转移。
Dev Growth Differ. 2022 Dec;64(9):558-565. doi: 10.1111/dgd.12824. Epub 2022 Nov 30.
2
Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation.通过电穿孔将双链RNA无创递送至脱蜡蜱卵内
PLoS One. 2015 Jun 19;10(6):e0130008. doi: 10.1371/journal.pone.0130008. eCollection 2015.
3
Single neuron electroporation in manipulating and measuring the central nervous system.用于操纵和测量中枢神经系统的单神经元电穿孔技术。
Int Arch Med. 2010 Nov 5;3:28. doi: 10.1186/1755-7682-3-28.
4
Single cell and neural process experimentation using laterally applied electrical fields between pairs of closely apposed microelectrodes with vertical sidewalls.利用垂直侧壁的成对紧密并置微电极之间横向施加的电场进行单细胞和神经突起实验。
Biosens Bioelectron. 2009 Aug 15;24(12):3600-7. doi: 10.1016/j.bios.2009.05.024. Epub 2009 May 27.
本文引用的文献
1
Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum.电转化水稻、小麦和高粱原生质体后，与植物病毒或果蝇启动子相连的外源基因的表达。
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6815-9. doi: 10.1073/pnas.83.18.6815.
2
Plasmid DNA and siRNA transfection of intestinal epithelial monolayers by electroporation.通过电穿孔法对肠上皮单层细胞进行质粒DNA和小干扰RNA转染。
Int J Pharm. 2006 Jun 6;315(1-2):122-33. doi: 10.1016/j.ijpharm.2006.02.022. Epub 2006 Mar 6.
3
Small interfering RNA (siRNA) delivery into monocyte-derived dendritic cells by electroporation.通过电穿孔将小分子干扰RNA（siRNA）导入单核细胞衍生的树突状细胞。
J Immunol Methods. 2006 Apr 20;311(1-2):139-52. doi: 10.1016/j.jim.2006.01.021. Epub 2006 Mar 6.
4
Electroporation of cells in microfluidic devices: a review.微流控设备中细胞的电穿孔：综述
Anal Bioanal Chem. 2006 Jun;385(3):474-85. doi: 10.1007/s00216-006-0327-3. Epub 2006 Mar 14.
5
Electroporation of neurons and growth cones in Aplysia californica.加州海兔神经元和生长锥的电穿孔
J Neurosci Methods. 2006 Mar 15;151(2):114-20. doi: 10.1016/j.jneumeth.2005.06.030. Epub 2005 Sep 19.
6
A novel electroporation device for gene delivery in large animals and humans.一种用于大型动物和人类基因递送的新型电穿孔装置。
Vaccine. 2006 May 22;24(21):4667-70. doi: 10.1016/j.vaccine.2005.08.068. Epub 2005 Sep 1.
7
In vivo analysis of mRNA stability using the Tet-Off system in the chicken embryo.利用鸡胚中的Tet-Off系统对mRNA稳定性进行体内分析。
Dev Biol. 2005 Aug 15;284(2):292-300. doi: 10.1016/j.ydbio.2005.05.021.
8
Ex ovo electroporation for gene transfer into older chicken embryos.用于将基因导入老龄鸡胚胎的卵外电穿孔法。
Dev Dyn. 2005 Aug;233(4):1470-7. doi: 10.1002/dvdy.20454.
9
Electroporation, an alternative to biolistics for transfection of Bombyx mori embryos and larval tissues.电穿孔法，一种用于家蚕胚胎和幼虫组织转染的基因枪注射法的替代方法。
J Insect Sci. 2003;3:17. doi: 10.1093/jis/3.1.17. Epub 2003 Jun 27.
10
Effective gene delivery to adult neurons by a modified form of electroporation.通过改良的电穿孔形式将基因有效递送至成年神经元。
J Neurosci Methods. 2005 Mar 15;142(1):137-43. doi: 10.1016/j.jneumeth.2004.08.012.
Zotero 插件