电穿孔：应用的武器库。
Electroporation: an arsenal of application.
机构信息
Department of Biological Science and Biotechnology, Life Science School, Sun Yat-Sen (ZhongShan) University, P.O. Box A075#, XinGangXi Road 135, HaiZhu District, 510275, Guangzhou, China,
出版信息
Cytotechnology. 2007 Jun;54(2):71-6. doi: 10.1007/s10616-007-9082-3. Epub 2007 Jun 16.
Abstract
Electroporation is a way to induce nanometersized membrane pore for exogenous substances delivery into cytoplasm using an artificial electric field. Now it was widely used for molecules transfer especially in molecular experiments and genetic aspects. In recent years, modern electroporation on the embryo was developed, whose most important point is that it adopts low energy and rectangular pulse that could obtain high transfection efficiency and low damage to the embryo. This paper reviewed on the pool of application: from lab works to human clinical treatments.
摘要
电穿孔是一种利用人工电场诱导纳米级细胞膜孔，将外源物质递送到细胞质中的方法。现在它被广泛用于分子转移，特别是在分子实验和遗传方面。近年来，胚胎的现代电穿孔技术得到了发展，其最重要的一点是采用低能量和矩形脉冲，从而可以获得高转染效率和低胚胎损伤。本文综述了其应用范围：从实验室工作到人类临床治疗。
相似文献
1
Electroporation: an arsenal of application.
Cytotechnology. 2007 Jun;54(2):71-6. doi: 10.1007/s10616-007-9082-3. Epub 2007 Jun 16.
2
High efficiency, site-specific transfection of adherent cells with siRNA using microelectrode arrays (MEA).
J Vis Exp. 2012 Sep 13(67):e4415. doi: 10.3791/4415.
3
Numerical study of gene electrotransfer efficiency based on electroporation volume and electrophoretic movement of plasmid DNA.
Biomed Eng Online. 2018 Jun 18;17(1):80. doi: 10.1186/s12938-018-0515-3.
4
Gain- and loss-of-function approaches in the chick embryo.
Methods Cell Biol. 2008;87:237-56. doi: 10.1016/S0091-679X(08)00212-4.
5
Response characteristics and optimization of electroporation: simulation based on finite element method.
Electromagn Biol Med. 2021 Jul 3;40(3):321-337. doi: 10.1080/15368378.2021.1951484. Epub 2021 Jul 18.
6
Avoiding the side effects of electric current pulse application to electroporated cells in disposable small volume cuvettes assures good cell survival.
Cell Mol Biol Lett. 2017 Jan 13;22:1. doi: 10.1186/s11658-016-0030-0. eCollection 2017.
7
Gene transfer to skeletal muscle by site-specific delivery of electroporation and ultrasound.
Biochem Biophys Res Commun. 2012 Jul 27;424(2):203-7. doi: 10.1016/j.bbrc.2012.06.090. Epub 2012 Jun 26.
8
Correlation between the loss of intracellular molecules and cell viability after cell electroporation.
Bioelectrochemistry. 2020 Oct;135:107550. doi: 10.1016/j.bioelechem.2020.107550. Epub 2020 May 13.
9
Microtrap array on a chip for localized electroporation and electro-gene transfection.
Bioelectrochemistry. 2022 Oct;147:108197. doi: 10.1016/j.bioelechem.2022.108197. Epub 2022 Jun 28.
10
Electroporation of subcutaneous mouse tumors by rectangular and trapezium high voltage pulses.
Bioelectrochemistry. 2004 Apr;62(1):83-93. doi: 10.1016/j.bioelechem.2003.11.001.
引用本文的文献
1
In vitro and in vivo gene transfer in the cloudy catshark Scyliorhinus torazame.
Dev Growth Differ. 2022 Dec;64(9):558-565. doi: 10.1111/dgd.12824. Epub 2022 Nov 30.
2
Non-Invasive Delivery of dsRNA into De-Waxed Tick Eggs by Electroporation.
PLoS One. 2015 Jun 19;10(6):e0130008. doi: 10.1371/journal.pone.0130008. eCollection 2015.
3
Single neuron electroporation in manipulating and measuring the central nervous system.
Int Arch Med. 2010 Nov 5;3:28. doi: 10.1186/1755-7682-3-28.
4
Single cell and neural process experimentation using laterally applied electrical fields between pairs of closely apposed microelectrodes with vertical sidewalls.
Biosens Bioelectron. 2009 Aug 15;24(12):3600-7. doi: 10.1016/j.bios.2009.05.024. Epub 2009 May 27.
本文引用的文献
1
Expression of a foreign gene linked to either a plant-virus or a Drosophila promoter, after electroporation of protoplasts of rice, wheat, and sorghum.
Proc Natl Acad Sci U S A. 1986 Sep;83(18):6815-9. doi: 10.1073/pnas.83.18.6815.
2
Plasmid DNA and siRNA transfection of intestinal epithelial monolayers by electroporation.
Int J Pharm. 2006 Jun 6;315(1-2):122-33. doi: 10.1016/j.ijpharm.2006.02.022. Epub 2006 Mar 6.
3
Small interfering RNA (siRNA) delivery into monocyte-derived dendritic cells by electroporation.
J Immunol Methods. 2006 Apr 20;311(1-2):139-52. doi: 10.1016/j.jim.2006.01.021. Epub 2006 Mar 6.
4
Electroporation of cells in microfluidic devices: a review.
Anal Bioanal Chem. 2006 Jun;385(3):474-85. doi: 10.1007/s00216-006-0327-3. Epub 2006 Mar 14.
5
Electroporation of neurons and growth cones in Aplysia californica.
J Neurosci Methods. 2006 Mar 15;151(2):114-20. doi: 10.1016/j.jneumeth.2005.06.030. Epub 2005 Sep 19.
6
A novel electroporation device for gene delivery in large animals and humans.
Vaccine. 2006 May 22;24(21):4667-70. doi: 10.1016/j.vaccine.2005.08.068. Epub 2005 Sep 1.
7
In vivo analysis of mRNA stability using the Tet-Off system in the chicken embryo.
Dev Biol. 2005 Aug 15;284(2):292-300. doi: 10.1016/j.ydbio.2005.05.021.
8
Ex ovo electroporation for gene transfer into older chicken embryos.
Dev Dyn. 2005 Aug;233(4):1470-7. doi: 10.1002/dvdy.20454.
9
Electroporation, an alternative to biolistics for transfection of Bombyx mori embryos and larval tissues.
J Insect Sci. 2003;3:17. doi: 10.1093/jis/3.1.17. Epub 2003 Jun 27.
10
Effective gene delivery to adult neurons by a modified form of electroporation.
J Neurosci Methods. 2005 Mar 15;142(1):137-43. doi: 10.1016/j.jneumeth.2004.08.012.
Zotero 插件