鉴定核糖体蛋白 S3a 为细胞核中新型 PI 3-激酶靶标。
Identification of ribosomal protein S3a as a candidate for a novel PI 3-kinase target in the nucleus.
机构信息
Laboratory of Biological Chemistry, Department of Applied Biological Chemistry, Graduate School of Agriculture and Life Science, University of Tokyo, Yayoi, Bunkyo-ku, Tokyo, Japan.
出版信息
Cytotechnology. 2002 Nov;40(1-3):85-92. doi: 10.1023/A:1023970222898.
Phosphatidylinositol 3,4,5-trisphosphate (PIP(3)) is an important lipid second messenger that mediates various cell responses. We have searched for the nuclear PIP(3) binding proteins using PIP(3) analogue beads. A 33 kD protein was detected in this method, which was identified as ribosomal protein S3a by the mass spectrometric analysis. The recombinant S3a protein bound specifically to PIP(3). S3a localized not only in the cytosol but also in the nucleus. Interestingly, not cytosolic but nuclear S3a bound to PIP(3), suggesting different roles of S3a in the cytosol and the nucleus.
磷脂酰肌醇 3,4,5-三磷酸（PIP(3)）是一种重要的脂质第二信使，介导各种细胞反应。我们使用 PIP(3)类似物珠搜索核 PIP(3)结合蛋白。在这种方法中检测到一种 33 kD 的蛋白质，通过质谱分析鉴定为核糖体蛋白 S3a。重组 S3a 蛋白特异性结合 PIP(3)。S3a 不仅定位于细胞质中，也定位于细胞核中。有趣的是，与 PIP(3)结合的不是细胞质而是核 S3a，这表明 S3a 在细胞质和细胞核中具有不同的作用。
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