体外培养的星形胶质细胞系产生的重组大鼠促红细胞生成素的神经保护作用及其与中国仓鼠卵巢细胞产生的促红细胞生成素的比较研究。
In vitro neuroprotective action of recombinant rat erythropoietin produced by astrocyte cell lines and comparative studies with erythropoietin produced by Chinese hamster ovary cells.
机构信息
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan.
出版信息
Cytotechnology. 1999 May;29(3):207-13. doi: 10.1023/A:1008014206833.
Abstract
In the central nervous system, astrocytes produce erythropoietin (Epo) and neurons express its receptor. To examine whether or not the brain Epo protects the in vitro cultured neurons from glutamate-induced cell death, we established rat astrocyte cell lines containing the plasmid for production of recombinant rat Epo. Epo partially purified from the culture medium showed a neuroprotective effect similar to that of rat Epo produced by Chinese hamster ovary (CHO) cells. Comparison was made in some other properties between Epo produced by these astrocyte cell lines and that by CHO cells. Digestion of Epo with glycosidases indicated that there was a little difference in glycosylation of Epo produced by two types of the cells.
摘要
在中枢神经系统中，星形细胞产生促红细胞生成素（Epo），神经元表达其受体。为了研究脑 Epo 是否能保护体外培养的神经元免受谷氨酸诱导的细胞死亡，我们建立了含有重组大鼠 Epo 生产质粒的大鼠星形细胞瘤系。从中培养的 Epo 部分纯化后，显示出与中国仓鼠卵巢（CHO）细胞产生的大鼠 Epo 相似的神经保护作用。在这两种细胞产生的 Epo 的一些其他特性方面进行了比较。用糖苷酶消化 Epo 表明，两种细胞产生的 Epo 的糖基化程度略有不同。
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