International Center for Biotechnology, Osaka University, 2-1, Yamada-oka, Suita, Osaka, 565-0871, Japan.
Cytotechnology. 2000 Oct;34(1-2):121-30. doi: 10.1023/A:1008157303025.
Spatial development of mouse bone marrow cellsemploying porous carriers was investigated in order todesign a bioreactor with a three-dimensionalhematopoietic microenvironment. Three types of porouscarriers were used for examining the spatialdevelopment of anchorage-dependent primary stromalcells as feeder cells. Stromal cells were found tospread well at a high density on a polyester nonwovendisc carrier (Fibra cel (FC)) under a scanningelectron microscope, while cells on porous cellulosebeads (Microcube (MC), 500 mum pore diameter)spread at a low density; cells on another type ofcellulose porous beads (CPB, 100 mum pore diameter)were globular. Mouse bone marrow cells wereinoculated to dishes containing three types of porouscarriers which shared more than 30% of the bottomsurface in a dish. The concentration of stromal cellsin the well containing FC was lower than that on theother two carriers. However, the weekly output oftotal hematopoietic cell (suspension cells) increasedbetween day 21 and 28 in the culture using FC while itdecreased monotonously in the cultures by use of theother two carriers. The proportion of progenitorcells (BFU-E, CFU-GM) in the total hematopoietic cellpopulation, after showing an initial decrease,increased after 1 week in the culture using FC whilethe proportion decreased monotonously to zero in thecultures using MC and CPB.
采用多孔载体研究了小鼠骨髓细胞的空间发育，以设计具有三维造血微环境的生物反应器。为了研究作为饲养细胞的锚定依赖性原代基质细胞的空间发育，使用了三种类型的多孔载体。在扫描电子显微镜下，发现基质细胞在聚酯无纺盘载体（Fibra cel（FC））上以高密度很好地扩散，而在多孔纤维素珠（Microcube（MC），500μm 孔径）上的细胞扩散密度较低；在另一种纤维素多孔珠（CPB，100μm 孔径）上的细胞呈球形。将小鼠骨髓细胞接种到含有三种多孔载体的培养皿中，这些载体在培养皿中共享超过 30%的底部表面。FC 孔中的基质细胞浓度低于其他两种载体。然而，在使用 FC 的培养中，悬浮细胞的总造血细胞（悬浮细胞）的每周产量在第 21 天至第 28 天之间增加，而在使用其他两种载体的培养中则单调下降。在使用 FC 的培养中，祖细胞（BFU-E、CFU-GM）在总造血细胞群体中的比例在最初下降后增加，而在 MC 和 CPB 的培养中则单调下降至零。
