Jung Hyosook, Tae Giyoong, Kim Young Ha, Johannsmann Diethelm
Research Center for Biomolecular Nanotechnology, Department of Materials Science and Engineering, Gwangju Institute of Science and Technology, 261 Cheomdan-gwagiro, Buk-gu, Gwangju 500-712, Republic of Korea.
Colloids Surf B Biointerfaces. 2009 Jan 1;68(1):111-9. doi: 10.1016/j.colsurfb.2008.09.025. Epub 2008 Oct 8.
Viscoelastic property and morphology of fibrin, which is caused by the enzymatic action of thrombin on fibrinogen, was studied quantitatively as a function of antithrombin III (AT) and heparin concentration by adding fibrinogen, heparin, AT, and thrombin, sequentially. A quartz crystal microbalance with impedance analysis (QCM-Z) was used to detect the change of viscoelastic properties as well as the thickness of adsorbed layer during fibrin formation process, while AFM was used to characterize the surface morphology of fibrin layer under the influence of two known anticoagulants. By the addition of fibrinogen initially, a rigid and thin fibrinogen layer with rather smooth surface morphology was formed on the substrate. Then, the addition of thrombin in the absence of AT and heparin, resulted in viscous and thick fibrin gel with textured surface morphology. As an anticoagulant, AT was added before the injection of thrombin, but AT in the absence of heparin showed only marginal effects at higher concentration of AT than that of thrombin. On the contrary, the thick and viscous fibrin layer was altered gradually to more fibrinogen-like layer as the heparin concentration increases at low concentrations of AT, demonstrating the powerful anticoagulant effect by heparin/AT complex. Interestingly, heparin alone without AT was also effective in preventing fibrin formation, influencing the viscoelastic property and morphology of fibrin.
通过依次添加纤维蛋白原、肝素、抗凝血酶III（AT）和凝血酶，定量研究了由凝血酶对纤维蛋白原的酶促作用引起的纤维蛋白的粘弹性性质和形态，该研究是作为抗凝血酶III（AT）和肝素浓度的函数进行的。使用带有阻抗分析的石英晶体微天平（QCM-Z）来检测纤维蛋白形成过程中粘弹性性质的变化以及吸附层的厚度，同时使用原子力显微镜（AFM）来表征在两种已知抗凝剂影响下纤维蛋白层的表面形态。最初添加纤维蛋白原时，在基底上形成了表面形态相当光滑的刚性薄纤维蛋白原层。然后，在不存在AT和肝素的情况下添加凝血酶，导致形成具有纹理表面形态的粘性厚纤维蛋白凝胶。作为抗凝剂，在注射凝血酶之前添加AT，但在不存在肝素的情况下，AT在高于凝血酶浓度时仅显示出微弱的效果。相反，在低浓度AT下，随着肝素浓度增加，厚而粘性的纤维蛋白层逐渐转变为更类似纤维蛋白原的层，这表明肝素/AT复合物具有强大的抗凝作用。有趣的是，单独使用不含AT的肝素也能有效防止纤维蛋白形成，影响纤维蛋白的粘弹性性质和形态。
