Song Weibo, Shao Chen, Yi Zhenzhen, Li Liqiong, Warren Alan, Al-Rasheid Khaled, Yang Jinpeng
Laboratory of Protozoology, Ocean University of China, Qingdao, China.
Eur J Protistol. 2009 Jan;45(1):38-50. doi: 10.1016/j.ejop.2008.06.002. Epub 2008 Nov 12.
The morphology, morphogenesis and small subunit rRNA (SSrRNA) gene sequence of a new marine euplotid ciliate, Diophrys apoligothrix spec. nov., isolated from a sandy beach near Qingdao, north China, were investigated. This organism is characterized as follows: rigid body with a sculptured surface; size in vivo about 60-110 x 40-70microm; adoral zone comprising about 28 membranelles; five frontal, two ventral, five transverse, one left marginal and three caudal cirri; five dorsal kineties with sparsely distributed dikinetids; two ellipsoid macronuclear nodules; marine habitat. Although the main morphogenetic events are similar to those reported in other congeners, three features are noteworthy: (1) the parental adoral zone is retained almost unchanged by the proter except for the outer margin of membranelles in the proximal portion, where the de-differentiation and then reorganization take place in situ in the mid-to-late phases of division; (2) the dorsal kinety anlagen are formed via a two-step mode; (3) the marginal cirrus anlage is formed de novo near the old structure and does not divide. Based on the morphological, morphogenetic and SSrRNA gene sequence data, it was concluded that D. apoligothrix is a well-outlined and distinctive member of the genus Diophrys.
对从中国北方青岛附近沙滩分离出的一种新的海洋真核纤毛虫——多毛双眉虫（Diophrys apoligothrix spec. nov.）的形态学、形态发生及小亚基核糖体RNA（SSrRNA）基因序列进行了研究。该生物具有以下特征：身体坚硬，表面有纹饰；活体大小约为60 - 110×40 - 70微米；口围带约由28片小膜组成；有5根额纤毛、2根腹纤毛、5根横纤毛、1根左缘纤毛和3根尾纤毛；5列背纤毛，双动基体分布稀疏；有2个椭圆形大核结节；生活在海洋环境中。尽管主要的形态发生事件与其他同属物种报道的相似，但有三个特征值得注意：（1）除近端部分小膜的外缘外，亲代口围带几乎未被前仔虫改变，在分裂中后期，该部分原位去分化然后重组；（2）背纤毛原基通过两步模式形成；（3）缘纤毛原基在旧结构附近重新形成，且不分裂。基于形态学、形态发生及SSrRNA基因序列数据，得出结论：多毛双眉虫是双眉虫属中一个轮廓清晰且独特的成员。
