同时检测抗丙型肝炎病毒抗体和丙型肝炎病毒核心抗原。

Simultaneous detection of anti-HCV antibody and HCV core antigen.

作者信息

Ansaldi Filippo, Icardi Giancarlo

机构信息

Department of Health Sciences, University of Genoa, Genoa, Italy.

出版信息

Methods Mol Biol. 2009;510:15-23. doi: 10.1007/978-1-59745-394-3_2.

DOI:10.1007/978-1-59745-394-3_2

PMID:19009250

Abstract

HCV infection is usually diagnosed by means of an enzyme immune assay for the detection of antibody against HCV. The window period between infection and seroconversion remains a dramatic problem in the transfusional and diagnostic setting. In this chapter, we report (i) procedures for assays using two different approaches designed to reduce the window period and (ii) performance in terms of specificity and sensitivity in the detection of both antibody and antigen, and we compare their efficacy with that of commercial assays.

摘要

丙型肝炎病毒（HCV）感染通常通过酶免疫测定法来检测抗HCV抗体进行诊断。在输血和诊断环境中，感染与血清转化之间的窗口期仍然是一个严重问题。在本章中，我们报告了（i）使用两种不同方法设计的检测程序，旨在缩短窗口期，以及（ii）在检测抗体和抗原方面的特异性和敏感性表现，并将它们的功效与商业检测方法进行比较。