O'Brien J K, Steinman K J, Robeck T R
SeaWorld and Busch Gardens Reproductive Research Center, San Diego, CA, USA.
Theriogenology. 2009 Jan 1;71(1):98-107. doi: 10.1016/j.theriogenology.2008.09.052. Epub 2008 Nov 17.
Efforts toward the conservation and captive breeding of wildlife can be enhanced by sperm sorting and associated reproductive technologies such as sperm cryopreservation and artificial insemination (AI). Sex ratio management is of particular significance to species which naturally exist in female-dominated social groups. A bias of the sex ratio towards females of these species will greatly assist in maintaining socially cohesive groups and minimizing male-male aggression. Another application of this technology potentially exists for endangered species, as the preferential production of females can enable propagation of those species at a faster rate. The particular assisted reproductive technology (ART) used in conjunction with sperm sorting for the production of offspring is largely determined by the quality and quantity of spermatozoa following sorting and preservation processes. Regardless of the ART selected, breeding decisions involving sex-sorted spermatozoa should be made in conjunction with appropriate genetic management. Zoological-based research on reproductive physiology and assisted reproduction, including sperm sorting, is being conducted on numerous terrestrial and marine mammals. The wildlife species for which the technology has undergone the most advance is the bottlenose dolphin. AI using sex-sorted fresh or frozen-thawed spermatozoa has become a valuable tool for the genetic and reproductive management of captive bottlenose dolphins with six pre-sexed calves, all of the predetermined sex born to date.
精子分选以及精子冷冻保存和人工授精(AI)等相关繁殖技术可加强野生动物保护和圈养繁殖工作。性别比例管理对于自然形成以雌性为主导的社会群体的物种尤为重要。这些物种的性别比例偏向雌性将极大有助于维持社会凝聚力群体并减少雄性间的攻击行为。这项技术对于濒危物种可能还有另一种应用,因为优先生产雌性后代能够使这些物种以更快的速度繁殖。与精子分选结合用于生产后代的特定辅助生殖技术(ART)在很大程度上取决于分选和保存过程后精子的质量和数量。无论选择何种辅助生殖技术,涉及按性别分选精子的繁殖决策都应结合适当的遗传管理来做出。目前正在对众多陆生和海洋哺乳动物开展基于动物园的生殖生理学和辅助生殖研究,包括精子分选。该技术发展最为先进的野生动物物种是宽吻海豚。使用按性别分选的新鲜或冻融精子进行人工授精已成为圈养宽吻海豚遗传和繁殖管理的一项宝贵工具,迄今已有六头预先确定性别的小牛出生。