KIOM-79可预防糖尿病db/db小鼠视网膜中的细胞凋亡及晚期糖基化终末产物的积累。
KIOM-79 prevents apoptotic cell death and AGEs accumulation in retinas of diabetic db/db mice.
作者信息
Sohn Eun Jin, Kim Young Sook, Kim Chan-Sik, Lee Yun Mi, Kim Jin Sook
机构信息
Department of Herbal Pharmaceutical Development, Korea Institute of Oriental Medicine, 461-24 Jeonmin-dong, Yuseong-gu, Daejeon 305-811, Republic of Korea.
出版信息
J Ethnopharmacol. 2009 Jan 12;121(1):171-4. doi: 10.1016/j.jep.2008.09.036. Epub 2008 Nov 1.
AIM OF THE STUDY
KIOM-79 retards the development of diabetic nephropathy in animal models of type 1 and type 2 diabetes. In this study, we evaluated whether KIOM-79 could prevent apoptotic cell death and advanced glycation end products (AGEs) accumulation in the retinas of diabetic db/db mice.
MATERIAL AND METHODS
Mice were treated orally with KIOM-79 (150 mg/kg body weight) once daily for 12 weeks. Levels of retinal ganglion cell death were measured by terminal dUTP nick-end labeling (TUNEL) assay. In the retina, the activity of caspase-3 (a marker of apoptosis) and the formation of AGEs were measured by immunohistochemical staining.
RESULTS
KIOM-79 reduced the number of TUNEL-immunoreactive retinal cells. KIOM-79 attenuated caspase-3 expression and AGEs accumulation in the retina.
CONCLUSIONS
These data show that KIOM-79 can prevent apoptosis in neuronal cells, AGEs accumulation in the retina, and retard the development of diabetic retinopathy.
研究目的
KIOM - 79可延缓1型和2型糖尿病动物模型中糖尿病肾病的发展。在本研究中，我们评估了KIOM - 79是否能预防糖尿病db/db小鼠视网膜中的凋亡性细胞死亡和晚期糖基化终末产物（AGEs）的积累。
材料与方法
小鼠每天口服一次KIOM - 79（150毫克/千克体重），持续12周。通过末端脱氧核苷酸转移酶介导的缺口末端标记（TUNEL）测定法测量视网膜神经节细胞死亡水平。在视网膜中，通过免疫组织化学染色测量半胱天冬酶 - 3（凋亡标志物）的活性和AGEs的形成。
结果
KIOM - 79减少了TUNEL免疫反应性视网膜细胞的数量。KIOM - 79减弱了视网膜中半胱天冬酶 - 3的表达和AGEs的积累。
结论
这些数据表明，KIOM - 79可以预防神经元细胞凋亡、视网膜中AGEs的积累，并延缓糖尿病视网膜病变的发展。
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