采用液相色谱-串联质谱法测定大鼠血浆中亲水性紫杉醇衍生物7-木糖基-10-去乙酰紫杉醇。
Determination of a hydrophilic paclitaxel derivative, 7-xylosyl-10-deacetylpaclitaxel in rat plasma by LC-MS/MS.
作者信息
Jiang Shou Gang, Zu Yuan Gang, Zhang Lin, Fu Yu Jie, Zhang Yu, Wang Zhou, Hua Xin, Wang Jing Tao
机构信息
Key Laboratory of Forest Plant Ecology, Ministry of Education, Northeast Forestry University, Harbin, People's Republic of China.
出版信息
Biomed Chromatogr. 2009 May;23(5):472-9. doi: 10.1002/bmc.1138.
A LC-MS/MS method for the determination of a hydrophilic paclitaxel derivative 7-xylosyl-10-deacetylpaclitaxel in rat plasma was developed to evaluate the pharmacokinetics of 7-xylosyl-10-deacetylpaclitaxel in the rats. 7-Xylosyl-10-deacetylpaclitaxel and docetaxel (IS for 7-xylosyl-10-deacetylpaclitaxel) were extracted from rat plasma with acetic ether and analyzed on a Hypersil C(18 )column (4.6 x 150 mm i.d., particle size 5 microm) with the mobile phase of ACN/0.05% formic acid (50:50, v/v). The analytes were detected using an ESI MS/MS in the multiple reaction monitoring mode. The standard curves for 7-xylosyl-10-deacetylpaclitaxel in plasma were linear (r > 0.999) over the concentration range of 2.0-1000 ng/mL with a weighting of 1/concentration(2). The method showed a satisfactory sensitivity (2.0 ng/mL using 50 microL plasma), precision (CV <or= 10.1%), accuracy (relative error -12.4 to 12.0%), and selectivity. This method was successfully applied to the pharmacokinetic study of 7-xylosyl-10-deacetylpaclitaxel in rat plasma after intravenous administration of 7-xylosyl-10-deacetylpaclitaxel to female Wistar rats.
建立了一种用于测定大鼠血浆中亲水性紫杉醇衍生物7-木糖基-10-去乙酰紫杉醇的液相色谱-串联质谱法，以评估7-木糖基-10-去乙酰紫杉醇在大鼠体内的药代动力学。用乙酸乙酯从大鼠血浆中提取7-木糖基-10-去乙酰紫杉醇和多西他赛（7-木糖基-10-去乙酰紫杉醇的内标），并在Hypersil C(18) 柱（内径4.6×150 mm，粒径5μm）上进行分析，流动相为乙腈/0.05%甲酸（50:50，v/v）。采用电喷雾串联质谱在多反应监测模式下检测分析物。血浆中7-木糖基-10-去乙酰紫杉醇的标准曲线在2.0-1000 ng/mL浓度范围内呈线性（r>0.999），权重为1/浓度(2)。该方法具有令人满意的灵敏度（使用50μL血浆时为2.0 ng/mL）、精密度（CV≤10.1%）、准确度（相对误差-12.4至12.0%）和选择性。将该方法成功应用于雌性Wistar大鼠静脉注射7-木糖基-10-去乙酰紫杉醇后大鼠血浆中7-木糖基-10-去乙酰紫杉醇的药代动力学研究。
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