Cong Wei-Tao, Hwang Sun-Young, Jin Li-Tai, Choi Jung-Kap
Research Institute of Drug Development, College of Pharmacy, Chonnam National University, Kwangju, South Korea.
Electrophoresis. 2008 Nov;29(21):4304-15. doi: 10.1002/elps.200800150.
A novel fluorescence-based method for protein staining on SDS polyacrylamide gel is described. In this method, proteins are stained using counterion (palmatine and SDS) staining solution, which is inexpensive, easy to perform, and does not involve a destaining step. Fixing and staining of proteins using the counterion protocol take less than an hour. As little as 2 ng of protein can be detected. Another interesting feature of the staining protocol described here is the compatibility with MALDI-TOF MS which shows a similar number of identification score and sequence coverage compared with those of SYPRO Ruby.
描述了一种基于荧光的新型十二烷基硫酸钠聚丙烯酰胺凝胶蛋白质染色方法。在该方法中，使用反离子（巴马汀和十二烷基硫酸钠）染色溶液对蛋白质进行染色，该溶液价格低廉、操作简便，且无需脱色步骤。使用反离子方案对蛋白质进行固定和染色耗时不到一小时。可检测低至2纳克的蛋白质。此处所述染色方案的另一个有趣特点是与基质辅助激光解吸电离飞行时间质谱兼容，与SYPRO Ruby相比，其鉴定得分数量和序列覆盖率相似。
