White Elizabeth J, Venter Mauritz, Hiten Nicholas F, Burger Johan T
Department of Genetics, Stellenbosch University, Matieland, South Africa.
Biotechnol J. 2008 Nov;3(11):1424-8. doi: 10.1002/biot.200800207.
A wide range of plant RNA extraction methods are available; however, many of these are limited in their application for a diverse range of plant species. With special emphasis on robustness and versatility, we have improved the cetyltrimethylammonium bromide (CTAB) method and isolated high-quality RNA from 16 different plant species. The major modifications made to the protocol described here were a reduction of sample treatment steps and an increase in beta-mercaptoethanol concentration (to 3%) resulting in a robust, rapid and reproducible plant RNA extraction protocol that can be used for a broad range of plant species and tissue types.
目前有多种植物RNA提取方法；然而，其中许多方法在应用于多种不同植物物种时存在局限性。特别强调方法的稳健性和通用性，我们改进了十六烷基三甲基溴化铵（CTAB）法，并从16种不同植物物种中分离出了高质量的RNA。对本文所述方案的主要改进包括减少样品处理步骤以及增加β-巯基乙醇浓度（至3%），从而得到了一种稳健、快速且可重复的植物RNA提取方案，该方案可用于多种植物物种和组织类型。
