乌拉圭分离出的耐异烟肼结核分枝杆菌菌株中KatG酶Ser315Thr替代的流行情况及传播情况
Prevalence and dissemination of the Ser315Thr substitution within the KatG enzyme in isoniazid-resistant strains of Mycobacterium tuberculosis isolated in Uruguay.
作者信息
Varela Gustavo, González Sabina, Gadea Pilar, Coitinho Cecilia, Mota Inés, González Gladys, Goñi Fernando, Rivas Carlos, Schelotto Felipe
机构信息
Department of Bacteriology and Virology, Institute of Hygiene, Universidad de la República, Av. Alfredo Navarro 3051, Montevideo, Uruguay.
Laboratory Department, Honorary Anti-Tuberculosis Commission, 18 de Julio 2175, Montevideo, Uruguay.
出版信息
J Med Microbiol. 2008 Dec;57(Pt 12):1518-1522. doi: 10.1099/jmm.0.2008/001917-0.
The aim of this study was to determine the prevalence of Ser315Thr substitution in isoniazid (INH)-resistant strains of Mycobacterium tuberculosis in Uruguay. The katG gene of 62 INH-resistant strains was analysed by an RFLP-PCR assay. PCR products were digested with MspI to detect Ser315Thr and Arg463Leu substitutions. A total of 16 of the 62 (26 %) INH-resistant strains analysed had a Ser315Thr substitution. Only one INH-resistant strain had an Arg463Leu substitution and two strains had a deletion in katG. Of the 16 strains with Ser315Thr, 15 showed different profiles using a double-repetitive-element PCR assay, demonstrating that there was no local dissemination of any particular strain. These findings are in agreement with published data from regions where the prevalence of tuberculosis (TB) is intermediate and may be due in part to the success of the local TB control programme.
本研究的目的是确定乌拉圭结核分枝杆菌异烟肼（INH）耐药菌株中Ser315Thr替代的流行情况。通过RFLP-PCR分析了62株INH耐药菌株的katG基因。用MspI消化PCR产物以检测Ser315Thr和Arg463Leu替代。在分析的62株INH耐药菌株中，共有16株（26%）发生了Ser315Thr替代。只有1株INH耐药菌株发生了Arg463Leu替代，2株katG基因存在缺失。在16株发生Ser315Thr替代的菌株中，15株使用双重复元件PCR分析显示出不同的图谱，表明没有任何特定菌株的局部传播。这些发现与结核病（TB）流行率处于中等水平地区的已发表数据一致，这可能部分归因于当地结核病控制项目的成功。
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