Kanazawa Y, Kitada M, Mori T, Inukai Y, Imaoka S, Funae Y, Kamataki T
Tenshi Junior College, Sapporo, Japan.
Mol Pharmacol. 1991 Apr;39(4):456-60.
Ascorbic acid (VC) deficiency resulted in a decrease in the activities of aminopyrine N-demethylase, aniline hydroxylase, and p-nitroanisole O-demethylase and in the content of cytochrome P-450, as spectrally determined, whereas it caused an increase in the activities of 6 beta-hydroxylases for testosterone and progesterone in liver microsomes of guinea pigs. Western blot analysis of liver microsomes with antibodies to rat P-448-H (P-4501A2), P-450j (P-450IIE), P-450 PB-1 (P-450IIIA), and P-450b (P-450IIB1) showed that VC deficiency decreased the amount of cytochrome P-450 immunochemically related to P-450IA2 and P-450IIE but did not change the amount of the form that was cross-reactive with antibodies to P-450IIB1 and tended to slightly increase (not statistically significantly) the amount of the form of the cytochrome immunochemically related to P-450IIIA. The larger decrease by VC deficiency in the amount of cytochrome P-450 that was cross-reactive to the rat P-450IA2 resulted in a lower capacity of liver microsomes to activate promutagens, such as 2-amino-3-methyl-imidazo(4,5-f)quinoline and aflatoxin B1. These results indicate that VC deficiency in guinea pigs differentially affects the content of individual forms of cytochrome P-450.
抗坏血酸（VC）缺乏导致氨基比林N - 脱甲基酶、苯胺羟化酶和对硝基苯甲醚O - 脱甲基酶的活性降低，以及通过光谱测定的细胞色素P - 450含量降低，而它却使豚鼠肝微粒体中睾酮和孕酮的6β - 羟化酶活性增加。用针对大鼠P - 448 - H（P - 4501A2）、P - 450j（P - 450IIE）、P - 450 PB - 1（P - 450IIIA）和P - 450b（P - 450IIB1）的抗体对肝微粒体进行蛋白质免疫印迹分析表明，VC缺乏降低了与P - 450IA2和P - 450IIE免疫化学相关的细胞色素P - 450的量，但未改变与P - 450IIB1抗体交叉反应的形式的量，并且倾向于使与P - 450IIIA免疫化学相关的细胞色素形式的量略有增加（无统计学显著性）。VC缺乏导致与大鼠P - 450IA2交叉反应的细胞色素P - 450量的更大减少，使得肝微粒体激活前诱变剂（如2 - 氨基 - 3 - 甲基 - 咪唑并(4,5 - f)喹啉和黄曲霉毒素B1）的能力降低。这些结果表明，豚鼠体内VC缺乏对细胞色素P - 450各形式的含量有不同影响。
