Roth Christopher C, Bell Cardin H, Woodson Benjamin, Schultz Andrew D, Palmer Blake W, Frimberger Dominic, Fung Kar-Ming, Lin Hsueh-Kung, Kropp Bradley P
Department of Urology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
BJU Int. 2009 Mar;103(6):836-41. doi: 10.1111/j.1464-410X.2008.08231.x. Epub 2008 Nov 19.
To determine if porcine small intestinal submucosa (SIS)-regenerated urothelium expresses markers of urothelial differentiation, uroplakin and zona occludens-1 (ZO-1), and whether their expression correlates with the histological appearance of the urothelium.
In all, 15 rats underwent partial cystectomy and bladder replacement with SIS. Regenerated bladders were harvested at either 2, 7, 14, 28, or 56 days after SIS grafting. Histological examination with haematoxylin and eosin staining was conducted to assess tissue regeneration. Immunohistochemistry was performed with uroplakin and ZO-1 antibodies.
By 14 days after SIS grafting, the urothelial layer was completely confluent over the SIS. Expression of uroplakin and ZO-1, evident at 2 days after SIS grafting, progressed from a cytoplasmic pattern of expression to a mature pattern of cytoplasmic and membrane expression by 56 days after SIS grafting.
In vivo tissue regeneration produces histologically and phenotypically mature urothelium within 2 weeks of SIS implantation. Regeneration of functional urothelium is probably essential for the subsequent development of the remaining bladder.
确定猪小肠黏膜下层（SIS）再生的尿路上皮是否表达尿路上皮分化标志物、尿桥蛋白和紧密连接蛋白1（ZO-1），以及它们的表达是否与尿路上皮的组织学外观相关。
总共15只大鼠接受了部分膀胱切除术并用SIS进行膀胱替代。在SIS移植后2、7、14、28或56天收获再生膀胱。进行苏木精和伊红染色的组织学检查以评估组织再生。用尿桥蛋白和ZO-1抗体进行免疫组织化学检测。
在SIS移植后14天，尿路上皮层在SIS上完全融合。尿桥蛋白和ZO-1的表达在SIS移植后2天就很明显，从细胞质表达模式发展到SIS移植后56天的细胞质和膜表达的成熟模式。
体内组织再生在SIS植入后2周内产生组织学和表型成熟的尿路上皮。功能性尿路上皮的再生可能对剩余膀胱的后续发育至关重要。
Zotero 插件