Nawa H, Nakanishi S, Patterson P H
Division of Biology, California Institute of Technology, Pasadena.
J Neurochem. 1991 Jun;56(6):2147-50. doi: 10.1111/j.1471-4159.1991.tb03479.x.
The cholinergic differentiation factor (CDF) in heart cells is identical to leukemia inhibitory factor (LIF). Recombinant CDF/LIF was shown to alter dramatically neurotransmitter production as well as the levels of several neuropeptides in cultured rat sympathetic neurons. Here it is shown that these changes are likely to be caused by alterations in the mRNA for these proteins and peptides. Growth in 1 nM recombinant CDF/LIF induces mRNA for acetyl CoA: choline-O-acetyltransferase [EC 2.3.1.6; choline acetyltransferase (ChAT)], somatostatin (SOM), substance P, and vasoactive intestinal polypeptide while lowering mRNA levels of tyrosine hydroxylase (EC 1.14.16.2) and neuropeptide Y (NPY). In addition, the sizes of the mRNAs for ChAT, SOM, and NPY are larger after recombinant CDF/LIF treatment.
心脏细胞中的胆碱能分化因子（CDF）与白血病抑制因子（LIF）相同。已证明重组CDF/LIF可显著改变培养的大鼠交感神经元中神经递质的产生以及几种神经肽的水平。本文表明，这些变化可能是由这些蛋白质和肽的mRNA改变引起的。在1 nM重组CDF/LIF中生长可诱导乙酰辅酶A：胆碱-O-乙酰转移酶[EC 2.3.1.6；胆碱乙酰转移酶（ChAT）]、生长抑素（SOM）、P物质和血管活性肠肽的mRNA表达，同时降低酪氨酸羟化酶（EC 1.14.16.2）和神经肽Y（NPY）的mRNA水平。此外，重组CDF/LIF处理后，ChAT、SOM和NPY的mRNA大小更大。
