良性、低恶性潜能及低级别浆液性上皮性卵巢肿瘤的免疫组织化学分析

Immunohistochemical profiling of benign, low malignant potential and low grade serous epithelial ovarian tumors.

作者信息

Ouellet Véronique, Ling Tak Hay, Normandin Karine, Madore Jason, Lussier Christian, Barrès Véronique, Bachvarov Dimcho, Rancourt Claudine, Tonin Patricia N, Provencher Diane M, Mes-Masson Anne-Marie

机构信息

Centre de recherche du centre hospitalier de l'Université de Montréal/Institut du cancer de Montréal, Montreal, Canada.

出版信息

BMC Cancer. 2008 Nov 26;8:346. doi: 10.1186/1471-2407-8-346.

DOI:10.1186/1471-2407-8-346

PMID:19032793

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2610034/

Abstract

BACKGROUND

Serous epithelial ovarian tumors can be subdivided into benign (BOV), low malignant potential (LMP) or borderline and invasive (TOV) tumors. Although the molecular characteristics of serous BOV, LMP and low grade (LG) TOV tumors has been initiated, definitive immunohistochemical markers to distinguish between these tumor types have not been defined.

METHODS

In the present study, we used a tissue array composed of 27 BOVs, 78 LMPs and 23 LG TOVs to evaluate the protein expression of a subset of selected candidates identified in our previous studies (Ape1, Set, Ran, Ccne1 and Trail) or known to be implicated in epithelial ovarian cancer disease (p21, Ccnb1, Ckd1).

RESULTS

Statistically significant difference in protein expression was observed for Ccnb1 when BOV tumors were compared to LMP tumors (p = 0.003). When BOV were compared to LG TOV tumors, Trail was significantly expressed at a higher level in malignant tumors (p = 0.01). Expression of p21 was significantly lower in LG tumors when compared with either BOVs (p = 0.03) or LMPs (p = 0.001). We also observed that expression of p21 was higher in LMP tumors with no (p = 0.02) or non-invasive (p = 0.01) implants compared to the LMP associated with invasive implants.

CONCLUSION

This study represents an extensive analyse of the benign and highly differentiated ovarian disease from an immunohistochemical perspective.

摘要

背景

浆液性上皮性卵巢肿瘤可细分为良性（BOV）、低恶性潜能（LMP）或交界性及浸润性（TOV）肿瘤。尽管已经开始研究浆液性BOV、LMP和低级别（LG）TOV肿瘤的分子特征，但尚未确定区分这些肿瘤类型的明确免疫组化标志物。

方法

在本研究中，我们使用了一个组织芯片，该芯片由27个BOV、78个LMP和23个LG TOV组成，以评估在我们之前的研究中确定的一组选定候选物（Ape1、Set、Ran、Ccne1和Trail）或已知与上皮性卵巢癌相关的蛋白表达（p21、Ccnb1、Ckd1）。

结果

当将BOV肿瘤与LMP肿瘤进行比较时，观察到Ccnb1蛋白表达存在统计学显著差异（p = 0.003）。当将BOV与LG TOV肿瘤进行比较时，Trail在恶性肿瘤中的表达水平显著更高（p = 0.01）。与BOV（p = 0.03）或LMP（p = 0.001）相比，LG肿瘤中p21的表达显著更低。我们还观察到，与伴有浸润性种植的LMP相比，无种植（p = 0.02）或非浸润性种植（p = 0.01）的LMP肿瘤中p21的表达更高。