Sun Jian-Jun, Liu Yong, Zhang Peng-Bo, Chen Xin-Lin, Guo Zhen-Yu, Zhang Jian-Shu, Yang Peng-Bo, Shi Wei
Department of Neurosurgery, Second Affiliated Hospital of Xi'an Jiaotong University College of Medicine, Xi'an 710004, China.
Nan Fang Yi Ke Da Xue Xue Bao. 2008 Nov;28(11):2022-5.
To examine the proliferation of the neural progenitor cells in the subventricular zone (SVZ) and around the hematoma after intracerebral hemorrhage (ICH) in adult rats.
ICH was induced by stereotactic injection of type VII collagenase into the corpus striatum of adult rats, followed by pulse or continuous intrapenitoneal injection of bromodeoxyuridine (Brdu) to label the proliferating cells. The rats were sacrificed on days 2, 7, 14 and 28 following the ICH for immunohistochemistry of the tissues in the SVZ and around the hemotoma to determine the number of Brdu- immunoreactive cells.
With pulse Brdu labeling, a significant increase in the number of Brdu-immunoreactive cells in the ipsilateral and contralateral tissues in the SVZ and around the hematoma was observed 2-14 days, and the cell number reached the maximum on day 7 after ICH as compared with that of the sham-operated group. With continuous Brdu injection, the increase was observed on day 14 after ICH, and till day 28, the Brdu-immunoreactive cells in the SVZ decreased to the control level, but some positive cells still persisted in the tissues around the hematoma.
ICH induces transient and regional increase in the cell proliferation in the ipilateral and contraletral SVZ and tissues around the hematoma, and the proliferating cells in the SVZ may migrate towards the hematoma area.
研究成年大鼠脑出血（ICH）后脑室下区（SVZ）及血肿周围神经祖细胞的增殖情况。
通过立体定向向成年大鼠纹状体注射VII型胶原酶诱导脑出血，随后脉冲或连续腹腔注射溴脱氧尿苷（Brdu）标记增殖细胞。在脑出血后第2、7、14和28天处死大鼠，对SVZ及血肿周围组织进行免疫组织化学检测，以确定Brdu免疫反应性细胞的数量。
脉冲Brdu标记后，在2 - 14天观察到SVZ同侧和对侧组织以及血肿周围Brdu免疫反应性细胞数量显著增加，与假手术组相比，ICH后第7天细胞数量达到最大值。连续注射Brdu后，ICH后第14天观察到细胞数量增加，直到第28天，SVZ中的Brdu免疫反应性细胞降至对照水平，但血肿周围组织中仍有一些阳性细胞。
ICH诱导同侧和对侧SVZ以及血肿周围组织中细胞增殖出现短暂性和区域性增加，且SVZ中的增殖细胞可能向血肿区域迁移。
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