Yang Tianlong, Liu Zhenbo, Liu Jiaming, Liu Haizhu, Huang Yahong, Liu Jianqin, Chen Xuebing, Zhao Yibing
Department of Chemistry and Key Laboratory of Analytical Sciences of the Ministry of Education, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2009 Feb;72(1):156-64. doi: 10.1016/j.saa.2008.09.023. Epub 2008 Oct 17.
The structures of multi-wall carbon nanotubes (MWNTs) were modified by H(2)SO(4)-HNO(3) and H(2)SO(4)-H(2)O(2), respectively. The corresponding products were water-soluble MWNTs-A and MWNTs-B. According to the experiment, it was found that MWNTs-B could emit stable solid substrate-room temperature phosphorescence (RTP) on the surface of paper with Ag(+) as perturber. Under the conditions of 70 degrees C and 15 min, MWNTs-B can react with Tween-80 and p-nitro-phenyl-fluorone (R) to form R-MWNTs-B-Tween-80 micellae compound, which could emit RTP of R and MWNTs-B on the surface of paper, respectively. Pb(2+) could cause the RTP of R and MWNTs-B enhanced sharply, respectively. DeltaI(p) is directly proportional to the content of Pb(2+). A new solid substrate-room temperature phosphorimetry (SS-RTP) for the determination of trace Pb(2+) has been established based on R-MWNTs-B-Tween-80 micellae compound containing double luminescent molecule. The detection limit of this method were 0.035 ag Pb(2+) spot(-1) (8.8 x 10(-17) g Pb(2+) ml(-1), MWNTs-B) and 0.028 ag Pb(2+) spot(-1) (7.1 x 10(-17) g Pb(2+) ml(-1), R). This method is of high sensitivity, good selectivity, high precision and accuracy. It could be applied to determine trace Pb(2+) in serum samples at wavelength of 453.7/623.0 nm (R) or 475.9/645.0 nm (MWNTs-B) with satisfactory results, showing that SS-RTP has flexibility and utility value. Simultaneously, this method can be used to diagnose human diseases. The reaction mechanism for the determination of trace Pb(2+) by SS-RTP based on R-MWNTs-B-Tween-80 micellae compound containing double luminescent molecule was also discussed.
分别用H₂SO₄-HNO₃和H₂SO₄-H₂O₂对多壁碳纳米管（MWNTs）的结构进行了修饰。相应产物为水溶性MWNTs-A和MWNTs-B。实验发现，MWNTs-B在以Ag⁺为干扰剂的情况下，能在滤纸表面发射稳定的固体基质室温磷光（RTP）。在70℃、15分钟的条件下，MWNTs-B能与吐温-80和对硝基苯基荧光酮（R）反应形成R-MWNTs-B-吐温-80胶束化合物，该化合物能分别在滤纸表面发射R和MWNTs-B的RTP。Pb²⁺能使R和MWNTs-B的RTP分别急剧增强。ΔI(p)与Pb²⁺含量成正比。基于含双发光分子的R-MWNTs-B-吐温-80胶束化合物，建立了一种测定痕量Pb²⁺的新型固体基质室温磷光法（SS-RTP）。该方法的检测限分别为0.035 ag Pb²⁺斑点⁻¹（8.8×10⁻¹⁷ g Pb²⁺ ml⁻¹，MWNTs-B）和0.028 ag Pb²⁺斑点⁻¹（7.1×10⁻¹⁷ g Pb²⁺ ml⁻¹，R）。该方法具有灵敏度高、选择性好、精密度和准确度高的特点。可用于在453.7/623.0 nm（R）或475.9/645.0 nm（MWNTs-B）波长下测定血清样品中的痕量Pb²⁺，结果令人满意，表明SS-RTP具有灵活性和实用价值。同时，该方法可用于人类疾病诊断。还讨论了基于含双发光分子的R-MWNTs-B-吐温-80胶束化合物的SS-RTP测定痕量Pb²⁺的反应机理。
