6,7-二甲基鲁米诺嗪作为一种潜在配体，用于选择性识别DNA双链中无碱基位点对面的腺嘌呤。

6,7-Dimethyllumazine as a potential ligand for selective recognition of adenine opposite an abasic site in DNA duplexes.

作者信息

Ye Zhiqiang, Rajendar Burki, Qing Dai, Nishizawa Seiichi, Teramae Norio

机构信息

Department of Chemistry, Graduate School of Science, Tohoku University, Aoba-ku, Sendai, 980-8578, Japan.

出版信息

Chem Commun (Camb). 2008 Dec 28(48):6588-90. doi: 10.1039/b816876h. Epub 2008 Nov 12.

DOI:10.1039/b816876h

PMID:19057788

Abstract

6,7-Dimethyllumazine more selectively binds to adenine (A) base opposite the abasic site in DNA duplexes (5'-TCC AGX[combining low line] GCA AC-3'/3'-AGG TCN CGT TG-5', X = AP site (Spacer C3), N= A, T, C and G) than the other three nucleobases with a dissociation constant K(d) of ca. 1.0 microM; substituted methyl groups enhance the binding affinity to A and the selectivity for A over T, compared to the parent molecule, lumazine.

摘要

与其他三种核碱基相比，6,7-二甲基鲁米诺嗪更具选择性地结合于DNA双链体（5'-TCC AGX[下划线]GCA AC-3'/3'-AGG TCN CGT TG-5'，X = 无碱基位点（间隔物C3），N = A、T、C和G）中与无碱基位点相对的腺嘌呤（A）碱基，其解离常数K(d)约为1.0微摩尔；与母体分子鲁米诺嗪相比，取代的甲基增强了对A的结合亲和力以及对A相对于T的选择性。