Tyring S K, Cauda R, Tumbarello M, Ortona L, Kennedy R C, Chanh T C, Kanda P
Department of Microbiology, University of Texas Medical Branch, Galveston.
Viral Immunol. 1991 Spring;4(1):33-42. doi: 10.1089/vim.1991.4.33.
Patients with human immunodeficiency virus (HIV) infections have aberrant production of a number of lymphokines and monokines. Envelope glycoproteins are believed to be important in HIV pathogenesis and may influence the production of these cytokines. Therefore, synthetic peptides corresponding to amino acid sequences 735-752 and 846-860 of glycoprotein gp41 and to amino acid sequence 304-328 of gp120 were investigated for their abilities to affect the production of the following cytokines by normal peripheral blood mononuclear cells in the presence of appropriate inducers: interferon (IFN)-alpha, IFN-gamma, interleukin (IL)-1, IL-2, and tumor necrosis factor (TNF). In contrast to cells and inducers alone (or in the presence of a control peptide), gp41 or gp120 synthetic peptides were able to depress the production of IFN-alpha, IFN-gamma and IL-2. In contrast, these peptides produced an elevation of the production of IL-1 and TNF. The effect of the gp41 peptides was more marked than that of gp120 peptides in most cases. These studies indicate that these HIV envelope glycoproteins may be directly responsible for aberrant lymphokine and monokine production in patients infected with this virus and therefore may be at least partially responsible for the pathogenesis of AIDS.
感染人类免疫缺陷病毒（HIV）的患者会出现多种淋巴因子和单核因子的异常产生。包膜糖蛋白被认为在HIV发病机制中起重要作用，并且可能影响这些细胞因子的产生。因此，研究了与糖蛋白gp41的氨基酸序列735 - 752和846 - 860以及gp120的氨基酸序列304 - 328相对应的合成肽，在存在适当诱导剂的情况下，它们影响正常外周血单个核细胞产生以下细胞因子的能力：干扰素（IFN）-α、IFN-γ、白细胞介素（IL）-1、IL-2和肿瘤坏死因子（TNF）。与单独的细胞和诱导剂（或存在对照肽时）相比，gp41或gp120合成肽能够抑制IFN-α、IFN-γ和IL-2的产生。相反，这些肽会使IL-1和TNF的产生增加。在大多数情况下，gp41肽的作用比gp120肽更明显。这些研究表明，这些HIV包膜糖蛋白可能直接导致感染该病毒的患者出现异常的淋巴因子和单核因子产生，因此可能至少部分地导致了艾滋病的发病机制。
