稻瘟病菌稻瘟菌中氧甾醇结合蛋白同源物MgORP1的特性分析
[Characterization of oxysterol binding protein homolog MgORP1 in the rice blast fungus Magnaporthe grisea].
作者信息
Chunhua Lin, Fucong Zheng
机构信息
Environment and Plant Protection College, Hainan University, Danzhou 571737, China.
出版信息
Wei Sheng Wu Xue Bao. 2008 Sep;48(9):1160-7.
OBJECTIVE
To study the component of ORPs (Oxysterol-binding proteins-related proteins) family in the rice fungus (Magnaporthe grisea), and the functions of MgORP1 gene with growing development of M. grisea, we constructed two MgORP1 deletion mutants and a complementation mutant.
METHODS
All proteins of ORPs have a conserved OSBP-related ligand-binding domain (ORD), which was compared by BLASTP with the sequences of M. grisea genomic DNA published in M. grisea database. One of ORP gene, designated as MgORP1, was deleted by gene replacement and then the phenotypes of delta MgORP1 were characterized.
RESULTS
Six putative ORP family proteins have been found in M. grisea, and the growth rate of hyphal colony in complete medium and conidiation of delta MgORP1 were lower, whereas the ability of conidia germination, appressoria formation and pathogenicity were normal.
CONCLUSION
MgORP1 gene of M. grisea was probably involved in colony growth and conidiation.
目的
为研究稻瘟病菌（Magnaporthe grisea）中氧化固醇结合蛋白相关蛋白（ORPs）家族的组成，以及MgORP1基因在稻瘟病菌生长发育过程中的功能，我们构建了两个MgORP1缺失突变体和一个互补突变体。
方法
ORPs的所有蛋白都有一个保守的OSBP相关配体结合结构域（ORD），通过BLASTP将其与稻瘟病菌数据库中公布的稻瘟病菌基因组DNA序列进行比对。通过基因替换缺失了一个ORP基因，命名为MgORP1，然后对ΔMgORP1的表型进行了鉴定。
结果
在稻瘟病菌中发现了6个推定的ORP家族蛋白，ΔMgORP1在完全培养基中的菌丝菌落生长速率和产孢量较低，而分生孢子萌发、附着胞形成和致病性能力正常。
结论
稻瘟病菌的MgORP1基因可能参与菌落生长和产孢。
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