Zujiao Fu, Yunjun Sun, Liqiu Xia, Xuezhi Ding, Shengbiao Hu, Wenping Li, Youming Zhang
Key Laboratory of Molecular Microbiology of Hunan Province, College of Life Science, Hunan Normal University, Changsha 410081, China.
Wei Sheng Wu Xue Bao. 2008 Sep;48(9):1250-5.
To localize and identify the insecticidal crystal protein genes (cry genes) in high-toxic Bacillus thuringiensis strain 4.0718.
The genomic DNA of B. thuringiensis strain 4.0718 was isolated by pulse field gel electrophoresis (PFGE), and the PFGE pattern and plasmid pattern were characterized. The cry genes in the strain were analyzed by Southern blot and the cry gene types on the chromosome and plasmid were identified by PCR and restriction fragment length polymorphism patterns of the PCR-amplified fragments (PCR-RFLP).
Southern blots showed that the cry genes in B. thuringiensis strain 4.0718 are present on both chromosome and plasmid. PCR and RFLP patterns showed that the chromosome and plasmid contain four cry genes, cry1Aa, crylAc, cry2Aa, and cry2Ab. However, the cry genes on the chromosome may be incomplete, while the cry1Aa and cry1Ac genes localized on the plasmid were found to contain the full coding sequence.
The abundant cry genes were first found on chromosome of B. thuringiensis strain 4.0718, and the cry-type genes are the same as the plasmid.
定位并鉴定高毒力苏云金芽孢杆菌菌株4.0718中的杀虫晶体蛋白基因（cry基因）。
采用脉冲场凝胶电泳（PFGE）分离苏云金芽孢杆菌菌株4.0718的基因组DNA，并对PFGE图谱和质粒图谱进行表征。通过Southern杂交分析该菌株中的cry基因，并通过PCR及PCR扩增片段的限制性片段长度多态性图谱（PCR-RFLP）鉴定染色体和质粒上的cry基因类型。
Southern杂交显示，苏云金芽孢杆菌菌株4.0718中的cry基因存在于染色体和质粒上。PCR和RFLP图谱表明，染色体和质粒含有4个cry基因，即cry1Aa、cry1Ac、cry2Aa和cry2Ab。然而，染色体上的cry基因可能不完整，而位于质粒上的cry1Aa和cry1Ac基因含有完整的编码序列。
首次在苏云金芽孢杆菌菌株4.0718的染色体上发现丰富的cry基因，且cry基因类型与质粒相同。
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