Senevirathna Dammika, Ranaweera Dinali, Abeysekera Dilshan, Kanakarathana Nalaka, De Silva Dharshan, Abeysundara Sachith, Samaraweera Preminda, Jayasinghe Saroj, Fernandopulle Neil
Genetech Molecular Diagnostics, No. 54, Kithulwatta Road, Colombo-08, Sri Lanka.
Southeast Asian J Trop Med Public Health. 2008 Nov;39(6):1054-6.
A total of 460 samples (serum or plasma) were obtained from clinically diagnosed liver disease patients from January 2006 to December 2007 and subjected to reverse transcription--polymerase chain reaction (RT-PCR) based detection of HCV. Of these, 32 samples (6.9%) were positive for HCV RNA. Samples that were positive for HCV were genotyped with type specific primers. The genotyping assay was validated by DNA sequencing and phylogenetic analysis. The Sri Lankan isolates were comprised predominantly of genotype 1b (46.9%) followed by genotype 2b (21.9%), 2a (15.6%) and mixed infection with genotypes 1b and 2b (3.1%). This is the first report of the distribution of HCV genotypes in Sri Lanka.
2006年1月至2007年12月期间，从临床诊断的肝病患者中总共采集了460份样本（血清或血浆），并采用基于逆转录 - 聚合酶链反应（RT-PCR）的方法检测丙型肝炎病毒（HCV）。其中，32份样本（6.9%）HCV RNA呈阳性。对HCV阳性的样本使用型特异性引物进行基因分型。基因分型检测通过DNA测序和系统发育分析进行验证。斯里兰卡的分离株主要由1b型（46.9%）组成，其次是2b型（21.9%）、2a型（15.6%）以及1b型和2b型的混合感染（3.1%）。这是关于斯里兰卡HCV基因型分布的首次报告。
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