[微小RNA143表达对结肠癌细胞增殖的影响]
[Effect of microRNA143 expression on cell proliferation in colonic carcinoma].
作者信息
Liu Hong, Zhang Su-Zhan, Cai Shan-Rong, Peng Jia-Ping, Zheng Shu
机构信息
Cancer Institute, The Second Affiliated Hospital, Zhejiang University School of Medicine, Hangzhou 310006, China.
出版信息
Zhonghua Zhong Liu Za Zhi. 2008 Jul;30(7):498-501.
OBJECTIVE
To investigate the effect of microRNA143 on cell proliferation and K-ras expression in colorectal carcinoma.
METHODS
Northern blot was used to examine the expression of miR-143 in colorectal carcinoma and adjacent normal tissues. A miR-143 expression vector was constructed and transfected into a human colon adenocarcinoma cell line SW480. Cell proliferation was evaluated by MTT assay. RT-PCR and Western blot were used to examine the expression of K-ras oncogene in transfected cells.
RESULTS
The level of mature miR-143 was lower in tumors compared with adjacent normal tissues in 81% of colorectal carcinoma specimens. In transfected cells, the increased accumulation of miR-143 inhibited the cell proliferation, and resulted in approximately 40.3% decrease of K-ras protein levels, but had no effect on level of K-ras mRNA.
CONCLUSION
The increased accumulation of miR-143 inhibits the proliferation of transfected cells, and results in down-regulation of K-ras protein in colorectal carcinoma.
目的
研究微小RNA143对结直肠癌细胞增殖及K-ras表达的影响。
方法
采用Northern印迹法检测结直肠癌组织及癌旁正常组织中miR-143的表达。构建miR-143表达载体并转染人结肠腺癌细胞系SW480。采用MTT法评估细胞增殖情况。运用RT-PCR和Western印迹法检测转染细胞中K-ras癌基因的表达。
结果
在81%的结直肠癌标本中，肿瘤组织中成熟miR-143水平低于癌旁正常组织。在转染细胞中，miR-143积累增加抑制了细胞增殖，并导致K-ras蛋白水平下降约40.3%，但对K-ras mRNA水平无影响。
结论
miR-143积累增加抑制了转染细胞的增殖，并导致结直肠癌中K-ras蛋白表达下调。
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