[不育男性精子染色体异常与精子DNA碎片化分析]

[Analysis of sperm chromosomal abnormalities and sperm DNA fragmentation in infertile males].

作者信息

Qiu Yi, Wang Leiguang, Zhang Lihong, Yang Dantong, Zhang Aidong, Yu Jianchun

机构信息

Key Laboratory for Improving Birth Outcome Technique, Shandong Institute for Family Planning, Jinan, Shandong, 250002 P. R. China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Dec;25(6):681-5.

PMID:19065532

Abstract

OBJECTIVE

To investigate changes in sperm chromosome and sperm DNA integrity of infertile males.

METHODS

The level of DNA fragmentation was determined by Sperm Chromatin Dispersion (SCD) test in infertile males with idiopathic severe oligoasthenozoospermia (ISOA, n= 19), couples with unexplained recurrent miscarriage (URM, n= 38) and adult healthy fertile men (control group, n= 32). Multi-color fluorescence in situ hybridization (FISH) was performed with probes specific for chromosomes 13, 18, 21, X and Y in the control group (n= 5), the ISOA (n= 10) and the URM (n= 12).

RESULTS

Patients with ISOA and URM showed a significantly higher abnormality with total rate of 4.02% (n= 19) and 3.91%(n= 38) for chromosomes 13, 18 and 21, and 2.03%, 1.98% for chromosomes X and Y, respectively, in their spermatozoa compared to control (1.29% and 0.61%, P< 0.01). A significantly higher proportion of total sperm DNA fragmentation was detected in patients with ISOA (40.7%+/- 17.8%) and URM (22.1%+/- 10.3%) of sperm compared to the control group (12.1%+/- 5.2%, P< 0.01). Moreover, a positive correlation was found between the rate of sperm chromosomal aberration and the rate of sperm DNA fragmentation (gamma = 0.874, P< 0.01, n= 27). There were significant correlation between sperm DNA fragmentation and sperm density, sperm motility and abnormal sperm (gamma = - 0.571, gamma = - 0.616 and gamma = 0.637, respectively, P< 0.01).

CONCLUSION

The result indicates that spermatozoa from patients with ISOA and URM contain greater DNA fragmentation and chromosomal aneuploidy and may lead to male infertility. Screening for sperm DNA damage may provide useful information in the diagnosis of male idiopathic infertility.

摘要

目的

研究不育男性精子染色体及精子DNA完整性的变化。

方法

采用精子染色质扩散（SCD）试验检测特发性严重少弱精子症（ISOA，n = 19）不育男性、不明原因复发性流产（URM，n = 38）夫妇及成年健康有生育能力男性（对照组，n = 32）的DNA片段化水平。对对照组（n = 5）、ISOA组（n = 10）和URM组（n = 12）的精子，用针对13、18、21、X和Y染色体的特异性探针进行多色荧光原位杂交（FISH）。

结果

与对照组相比，ISOA组和URM组患者精子中13、18和21号染色体的异常率显著更高，分别为4.02%（n = 19）和3.91%（n = 38），X和Y染色体的异常率分别为2.03%、1.98%，而对照组分别为1.29%和0.61%（P < 0.01）。与对照组（12.1%±5.2%，P < 0.01）相比，ISOA组（40.7%±17.8%）和URM组（22.1%±10.3%）患者精子中总精子DNA片段化比例显著更高。此外，精子染色体畸变率与精子DNA片段化率之间呈正相关（γ = 0.874，P < 0.01，n = 27）。精子DNA片段化与精子密度、精子活力及异常精子之间存在显著相关性（γ分别为 - 0.571、 - 0.616和0.637，P < 0.01）。