[基于多重PCR技术建立的水产品中三种细菌性病原体快速检测方法]
[Rapid detection method of three types of bacterial pathogens in aquatic products established by multiplex PCR].
作者信息
Yang Xiaojuan, Wu Qingping, Zhang Jumei, Xu Xiaoke
机构信息
Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Institute of Microbiology, Guangzhou 510070, China.
出版信息
Wei Sheng Yan Jiu. 2008 Sep;37(5):602-5.
OBJECTIVE
To develop a rapid multiplex PCR (m-PCR) assay for simultaneously detection of three foodborne pathogens in aquatic products.
METHODS
The invasion protein gene (invA) of Salmonella spp., toxR gene (toxR) of Vibrio parahaemolyticus and invasion-associated protein p60 gene (iap) of Listeria monocytogenes were used as the gene targets.
RESULTS
The multiplex PCR assay could be specific and rapid, and the detection limits were 10 cfu/ml when the artificially contaminated aquatic products were incubated at 37 degrees C for 10 h.
CONCLUSION
The multiplex PCR assay developed in this study could provide a cost-effective supplement of conventional microbiological methods for routine monitoring of food.
目的
开发一种快速多重聚合酶链反应（m-PCR）检测方法，用于同时检测水产品中的三种食源性病原体。
方法
以沙门氏菌属的侵袭蛋白基因（invA）、副溶血性弧菌的toxR基因（toxR）和单核细胞增生李斯特菌的侵袭相关蛋白p60基因（iap）作为基因靶点。
结果
该多重聚合酶链反应检测方法具有特异性和快速性，当人工污染的水产品在37℃孵育10小时时，检测限为10 cfu/ml。
结论
本研究开发的多重聚合酶链反应检测方法可为常规食品监测的传统微生物学方法提供一种经济高效的补充。
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