El-Saharty Y S, Metwaly F H, Refaat M, El-Khateeb S Z
Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, El-Kasr El-Aini Street, ET-11562 Cairo, Egypt.
Talanta. 2007 Apr 30;72(2):675-81. doi: 10.1016/j.talanta.2006.11.040. Epub 2007 Jan 8.
Four polyvinyl chloride (PVC) membrane sensors for the determination of hyoscine butylbromide are described and characterized. The sensors are based on the use of the ion association complexes of hyoscine cation with ammonium reineckate counter anions as ion exchange sites in the PVC matrix. The membranes incorporate ion association complexes of hyoscine with dibutylsebathete (sensor 1), dioctylphthalate (sensor 2), nitrophenyl octyl ether (sensor 3) and beta-cyclodextrin (sensor 4). The performance characteristics of these sensors were evaluated according to IUPAC recommendations, which reveal a fast, stable and linear response for hyoscine over the concentration range of 10(-5)-10(-2)M for sensors 1 and 2 and 10(-6)-10(-2) for sensors 3 and 4 with cationic slopes of -53.19, -55.17, -51.44 and -51.51mV per concentration decade for the four sensors, respectively. The direct potentiometric determination of hyoscine butylbromide using the proposed sensors gave average recoveries % of 99.92+/-1.11, 99.93+/-1.00, 99.94+/-1.18 and 99.87+/-1.39 for the four sensors, respectively. The sensors are used for determination of hyoscine butylbromide in laboratory prepared mixtures, pharmaceutical formulations in combination with ketoprofen and in plasma. Validation of the method shows suitability of the proposed sensors for use in the quality control assessment of hyoscine butylbromide. The developed method was found to be simple, accurate and precise when compared with a reported HPLC method.
本文描述并表征了用于测定丁溴东莨菪碱的四种聚氯乙烯（PVC）膜传感器。这些传感器基于东莨菪碱阳离子与雷氏铵盐抗衡阴离子的离子缔合络合物，作为PVC基质中的离子交换位点。这些膜包含东莨菪碱与癸二酸二丁酯（传感器1）、邻苯二甲酸二辛酯（传感器2）、硝基苯基辛基醚（传感器3）和β-环糊精（传感器4）的离子缔合络合物。根据国际纯粹与应用化学联合会（IUPAC）的建议对这些传感器的性能特征进行了评估，结果表明，对于传感器1和2，在10⁻⁵ - 10⁻²M的浓度范围内，以及对于传感器3和4，在10⁻⁶ - 10⁻²M的浓度范围内，东莨菪碱具有快速、稳定和线性响应，四种传感器的阳离子斜率分别为每浓度十年 -53.19、 -55.17、 -51.44和 -51.51mV。使用所提出的传感器直接电位法测定丁溴东莨菪碱，四种传感器的平均回收率分别为99.92±1.11%、99.93±1.00%、99.94±1.18%和99.87±1.39%。这些传感器用于测定实验室制备的混合物、与酮洛芬联合的药物制剂以及血浆中的丁溴东莨菪碱。该方法的验证表明所提出的传感器适用于丁溴东莨菪碱的质量控制评估。与报道的高效液相色谱（HPLC）方法相比，所开发的方法简单、准确且精密。
