Ruohola H, Bremer K A, Baker D, Swedlow J R, Jan L Y, Jan Y N
Howard Hughes Medical Institute, University of California, San Francisco 94143-0724.
Cell. 1991 Aug 9;66(3):433-49. doi: 10.1016/0092-8674(81)90008-8.
Oogenesis in Drosophila involves specification of both germ cells and the surrounding somatic follicle cells, as well as the determination of oocyte polarity. We found that two neurogenic genes, Notch and Delta, are required in oogenesis. These genes encode membrane proteins with epidermal growth factor repeats and are essential in the decision of an embryonic ectodermal cell to take on the fate of neuroblast or epidermoblast. In oogenesis, mutation in either gene leads to an excess of posterior follicle cells, a cell fate change reminiscent of the hyperplasia of neuroblasts seen in neurogenic mutant embryos. Furthermore, the Notch mutation in somatic cells causes mislocalization of bicoid in the oocyte. These results suggest that the neurogenic genes Notch and Delta are involved in both follicle cell development and the establishment of anterior-posterior polarity in the oocyte.
果蝇的卵子发生涉及生殖细胞及其周围体细胞滤泡细胞的特化，以及卵母细胞极性的确定。我们发现两个神经发生基因，Notch和Delta，在卵子发生过程中是必需的。这些基因编码具有表皮生长因子重复序列的膜蛋白，并且在胚胎外胚层细胞决定成为神经母细胞或表皮母细胞的命运中起着至关重要的作用。在卵子发生过程中，任一基因的突变都会导致后部滤泡细胞过多，这种细胞命运的改变让人联想到在神经发生突变胚胎中看到的神经母细胞增生。此外，体细胞中的Notch突变会导致卵母细胞中双尾蛋白的定位错误。这些结果表明，神经发生基因Notch和Delta既参与滤泡细胞的发育，也参与卵母细胞前后极性的建立。
