Cook J L, Shaffer J B, Bewley G C
Department of Genetics, North Carolina State University, Raleigh.
Dev Genet. 1991;12(3):219-25. doi: 10.1002/dvg.1020120307.
The tissue-specific expression and isozymic composition of Drosophila sn-glycerol-3-phosphate dehydrogenase (GPDH) (EC 1.1.1.8) have been determined for a high-activity control line and two variant lines that alter either the temporal or systemic expression of GPDH through a reduction in rates of polypeptide synthesis. The temporal variant exhibits a reduction in enzyme levels in all larval tissues and in the adult abdomen, while levels of activity in the adult thorax are equal to the control line. Isozymic analyses of these tissues demonstrate that it is the GPDH-3 species that is reduced in a temporal and tissue-specific manner. In contrast, the systemic variant demonstrates a uniform reduction of all isozymic species in each tissue and developmental stage. Analyses of the tissues of F1 hybrid offspring of each variant line and appropriately marked electrophoretic variants demonstrate that the tissue-specific effects observed are due to cis-acting elements that are tightly linked to the structural gene.
已针对一个高活性对照品系以及两个变异品系，测定了果蝇甘油-3-磷酸脱氢酶（GPDH）（EC 1.1.1.8）的组织特异性表达和同工酶组成。这两个变异品系通过降低多肽合成速率，改变了GPDH的时间或全身表达。时间变异品系在所有幼虫组织和成虫腹部的酶水平降低，而成虫胸部的活性水平与对照品系相当。对这些组织的同工酶分析表明，以时间和组织特异性方式减少的是GPDH-3种类。相比之下，全身变异品系在每个组织和发育阶段的所有同工酶种类均呈现一致减少。对每个变异品系与适当标记的电泳变异体的F1杂交后代的组织分析表明，观察到的组织特异性效应是由于与结构基因紧密连锁的顺式作用元件所致。
