Oehmen A, Fradinho J, Serra S, Carvalho G, Capelo J L, Velizarov S, Crespo J G, Reis M A M
REQUIMTE/CQFB, Chemistry Dept, FCT, Universidade Nova de Lisboa, Caparica, Portugal.
J Hazard Mater. 2009 Jun 15;165(1-3):1040-8. doi: 10.1016/j.jhazmat.2008.10.094. Epub 2008 Nov 5.
Mercury (Hg) is the most highly toxic heavy metal, and must be removed from waterways to very low levels. Biologically mediated mercury removal is an emerging technology that has the potential to be robust, efficient and cost-effective. In this study, the impact of carbon source on the behaviour and microbial community composition of mixed microbial cultures was evaluated, and their performance was compared with a pure culture of Pseudomonas putida spi3. Glucose and acetate, two carbon sources that are commonly present in wastewaters, were chosen for this study. Distinct microbial populations were enriched with each carbon source. Glucose led to a more suitable microbial culture for Hg(2+) bioreduction that was able to reduce Hg(2+) at faster rates when compared to acetate. Furthermore, acetate consistently led to poorer process performance, irrespective of the microbial culture, possibly due to the formation of mercuric acetate complexes. It is proposed that glucose can be a more beneficial carbon source than acetate for the successful operation of Hg bioremediation systems.
汞（Hg）是毒性最强的重金属，必须从水道中去除至极低水平。生物介导的汞去除是一项新兴技术，具有强大、高效且经济高效的潜力。在本研究中，评估了碳源对混合微生物培养物行为和微生物群落组成的影响，并将其性能与恶臭假单胞菌spi3的纯培养物进行了比较。本研究选择了废水中常见的两种碳源葡萄糖和乙酸盐。每种碳源都富集了不同的微生物种群。与乙酸盐相比，葡萄糖导致形成了更适合汞（2+）生物还原的微生物培养物，能够以更快的速率还原汞（2+）。此外，无论微生物培养情况如何，乙酸盐始终导致工艺性能较差，这可能是由于形成了醋酸汞络合物。有人提出，对于汞生物修复系统的成功运行而言，葡萄糖可能是比乙酸盐更有益的碳源。
