The effect of gas-phase reactions on the quantitation of cyclic hydrazone libraries by electrospray ionization (ESI) mass spectrometry.

Using mass spectrometry coupled with LC analysis we report evidence of diastereomer dependent fragmentation and oligomerization reactions in the ionization of acyl-hydrazone-based libraries of cyclic oligomers. These effects can significantly affect the accuracy of MS-based quantitations, but also provide a venue for examining ionization effects in dynamic combinatorial libraries (DCLs).