Chen Zhanguang, Liu Guoliang, Chen Meizhen, Xu Benjie, Peng Yurui, Chen Maohuai, Wu Mingyao
Department of Chemistry, Shantou University, Shantou, China.
Talanta. 2009 Feb 15;77(4):1365-9. doi: 10.1016/j.talanta.2008.09.016. Epub 2008 Sep 18.
An in vitro screening model using resonance light scattering (RLS) technique with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reagent as the reactive probe to target cancer cell was firstly developed. In this model, MTT was reduced by viable cancer cells to produce a purple formazan. Cell viability was proportional to the number of formazan induced strong light scattering signal. The inhibition rate of anticancer drug was found to vary inversely with the H(22)-MTT system RLS intensity. So it was intuitive to see the sequence of the tumor suppressive activity of six anticancer drugs without data processing by RLS/MTT screening spectra. Compared with the traditional MTT method, this method has high sensitivity, low detection limit and quite intuitive screening results which were identical to those obtained from the MTT colorimetric assay.
首次建立了一种体外筛选模型，该模型使用共振光散射（RLS）技术，以3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑（MTT）试剂作为靶向癌细胞的反应探针。在该模型中，活癌细胞将MTT还原以产生紫色甲臜。细胞活力与甲臜诱导的强光散射信号的数量成正比。发现抗癌药物的抑制率与H(22)-MTT系统的RLS强度成反比。因此，通过RLS/MTT筛选光谱无需数据处理就能直观地看出六种抗癌药物的抑癌活性顺序。与传统的MTT方法相比，该方法具有高灵敏度、低检测限以及非常直观的筛选结果，且这些结果与MTT比色法获得的结果一致。
